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Potential roles for pattern molecule of PAMP-triggered immunity in improving crop cold tolerance

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Abstract

Key message

The application of flagellin 22 (flg22), the most widely studied PAMP, enhance crop cold tolerance. ICE1-CBF pathway and SA signaling is involved in the alleviation of cold injury by flg22 treatment.

Abstract

Pathogen infection cross-activates cold response and increase cold tolerance of host plants. However, it is not possible to use the infection to increase cold tolerance of field plants. Here flagellin 22 (flg22), the most widely studied PAMP (pathogen-associated molecular patterns), was used to mimic the pathogen infection to cross-activate cold response. Flg22 treatment alleviated the injury caused by freezing in Arabidopsis, oilseed and tobacco. In Arabidopsis, flg22 activated the expression of immunity and cold-related genes. Moreover, the flg22 induced alleviation of cold injury was lost in NahG transgenic line (SA-deficient), sid2-2 and npr1-1 mutant plants, and flg22-induced expression of cold tolerance-related genes, which indicating that salicylic acid signaling pathway is required for the alleviation of cold injury by flg22 treatment. In short flg22 application can be used to enhance cold tolerance in field via a salicylic acid-depended pathway.

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All data generated or analysed during this study are included in this published article and its supplementary information files.

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Funding

This work was financially supported by the National Natural Science Foundation of China (Grant numbers 31470365 and 31700216).

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Author notes

  1. Ye Jin and Za Khai Tuang have contributed equally.

Authors and Affiliations

  1. Hubei Key Laboratory of Genetic Regulation and Integrative Biology, School of Life Sciences, Central China Normal University, Wuhan, 430079, China

    Ye Jin, Za Khai Tuang, Yizhong Wang, Zhenjiang Wu & Wannian Yang

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  1. Ye Jin
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  2. Za Khai Tuang
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  3. Yizhong Wang
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  4. Zhenjiang Wu
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  5. Wannian Yang
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Contributions

WY conceived and designed research. ZT, YJ, YW and ZW conducted experiments. YJ wrote the manuscript. All authors read and approved the manuscript.

Corresponding author

Correspondence to Wannian Yang.

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The authors declare that they have no conflict of interest.

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Communicated by Ying-Tang Lu.

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Jin, Y., Tuang, Z.K., Wang, Y. et al. Potential roles for pattern molecule of PAMP-triggered immunity in improving crop cold tolerance. Plant Cell Rep 41, 337–345 (2022). https://doi.org/10.1007/s00299-021-02811-4

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  • DOI: https://doi.org/10.1007/s00299-021-02811-4

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