Skip to main content
SpringerLink
Log in

Selection of transformation-efficient barley genotypes based on TFA (transformation amenability) haplotype and higher resolution mapping of the TFA loci

  • Original Article
  • Published:
Plant Cell Reports Aims and scope Submit manuscript

Abstract

Key message

The genetic substitution of transformation amenability alleles from ‘Golden Promise’ can facilitate the development of transformation-efficient lines from recalcitrant barley cultivars.

Abstract

Barley (Hordeum vulgare) cv. ‘Golden Promise’ is one of the most useful and well-studied cultivars for genetic manipulation. In a previous report, we identified several transformation amenability (TFA) loci responsible for Agrobacterium-mediated transformation using the F2 generation of immature embryos, derived from ‘Haruna Nijo’ × ‘Golden Promise,’ as explants. In this report, we describe higher density mapping of these TFA regions with additional SNP markers using the same transgenic plants. To demonstrate the robustness of transformability alleles at the TFA loci, we genotyped 202 doubled haploid progeny from the cross ‘Golden Promise’ × ‘Full Pint.’ Based on SNP genotype, we selected lines having ‘Golden Promise’ alleles at TFA loci and used them for transformation. Of the successfully transformed lines, DH120366 came the closest to achieving a level of transformation efficiency comparable to ‘Golden Promise.’ The results validate that the genetic substitution of TFA alleles from ‘Golden Promise’ can facilitate the development of transformation-efficient lines from recalcitrant barley cultivars.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4

Similar content being viewed by others

References

  • Anand A, Krichevsky A, Schornack S, Lahaye T, Tzfira T, Tang Y, Citovsky V, Mysore KS (2007) Arabidopsis VIRE2 INTERACTING PROTEIN2 is required for Agrobacterium T-DNA integration in Plants. Plant Cell 19:1695–1708

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Cheng M, Lowe BA, Spencer TM, Ye X, Armstrong CL (2004) Factors influencing Agrobacterium-mediated transformation of monocotyledonous species. In Vitro Cell Dev Biol Plant 40:31–45

    Article  Google Scholar 

  • Cistué L, Ramos A, Castillo AM, Romagosa I (1994) Production of large number of doubled haploid plants from barley anthers pretreated with high concentrations of mannitol. Plant Cell Rep 13:709–712

    Article  PubMed  Google Scholar 

  • Close TJ, Bhat PR, Lonardi S, Wu Y, Rostoks N, Ramsay L, Druka A, Stein N, Svensson JT, Wanamaker S, Bozdag S, Roose ML, Moscou MJ, Chao S, Varshney RK, Szűcs P, Sato K, Hayes PM, Matthews DE, Kleinhofs A, Muehlbauer GJ, DeYoung J, Marshall DF, Madishetty K, Fenton RD, Condamine P, Graner A, Waugh R (2009) Development and implementation of high-throughput SNP genotyping in barley. BMC Genom 10:1–13

    Article  Google Scholar 

  • Cogan NOI, Newbury HJ, Oldacres AM, Lynn JR, Kearsey MJ, King GJ, Puddephat IJ (2004) Identification and characterization of QTL controlling Agrobacterium-mediated transient and stable transformation of Brassica oleracea. Plant Biotechnol J 2:59–69

    Article  CAS  PubMed  Google Scholar 

  • Comadran J, Kilian B, Russell J, Ramsay L, Stein N, Ganal M, Shaw P, Bayer M, Thomas W, Marshall D, Hedley P, Tondelli A, Pecchioni N, Francia E, Korzun V, Walther A, Waugh R (2012) Natural variation in a homolog of Antirrhinum CENTRORADIALIS contributed to spring growth habit and environmental adaptation in cultivated barley. Nat Genet 44:1388–1392

    Article  CAS  PubMed  Google Scholar 

  • Deng W, Casao MC, Wang P, Sato K, Hayes PM, Finnegan EJ, Trevaskis B (2015) Direct links between the vernalization response and other key traits of cereal crops. Nat Commun 6:5882

    Article  PubMed  Google Scholar 

  • Echávarri B, Cistué L (2016) Enhancement in androgenesis efficiency in barley (Hordeum vulgare L.) and bread wheat (Triticum aestivum L.) by the addition of dimethyl sulfoxide to the mannitol pretreatment medium. Plant Cell Tiss Organ Cult 125:11–22

    Article  Google Scholar 

  • Harwood WA (2012) Advances and remaining challenges in the transformation of barley and wheat. J Exp Bot 63:1791–1798

    Article  CAS  PubMed  Google Scholar 

  • Hensel G, Valkov V, Middlefell-Williams J, Kumlehn J (2008) Efficient generation of transgenic barley: The way forward to modulate plant–microbe interactions. J Plant Physiol 165:71–82

    Article  CAS  PubMed  Google Scholar 

  • Hisano H, Sato K (2016) Genomic regions responsible for amenability to Agrobacterium-mediated transformation in barley. Sci Rep 6:37505

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Ikeuchi M, Sugimoto K, Iwase A (2013) Plant callus: mechanisms of induction and repression. The Plant Cell 25:3159–3173

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Ikeuchi M, Ogawa Y, Iwase A, Sugimoto K (2016) Plant regeneration: cellular origins and molecular mechanisms. Development 143:1442–1451

    Article  CAS  PubMed  Google Scholar 

  • Iwata H, Ninomiya S (2006) AntMap: Constructing genetic linkage maps using an ant colony optimization algorithm. Breed Sci 56:371–377

    Article  Google Scholar 

  • Kumlehn J, Serazetdinova L, Hensel G, Becker D, Loerz H (2006) Genetic transformation of barley (Hordeum vulgare L.) via infection of androgenetic pollen cultures with Agrobacterium tumefaciens. Plant Biotechnol J 4:251–261

    Article  CAS  PubMed  Google Scholar 

  • Lowe K, Wu E, Wang N, Hoerster G, Hastings C, Cho M-J, Scelonge C, Lenderts B, Chamberlin M, Cushatt J, Wang L, Ryan L, Khan T, Chow-Yiu J, Hua W, Yu M, Banh J, Bao Z, Brink K, Igo E, Rudrappa B, Shamseer PM, Bruce W, Newman L, Shen B, Zheng P, Bidney D, Falco SC, Register JC III, Zhao Z-Y, Xu D, Jones TJ, Gordon-Kamm WJ (2016) Morphogenic regulators Baby boom and Wuschel improve monocot transformation. Plant Cell 28:1998–2015

    Article  CAS  PubMed Central  Google Scholar 

  • Lü B, Wu J-J, Fu D-l (2015) Constructing the barley model for genetic transformation in Triticeae. J Integr Agric 14:453–468

    Article  Google Scholar 

  • Mrízová K, Holasková E, Öz MT, Jiskrová E, Frébort I, Galuszka P (2014) Transgenic barley: a prospective tool for biotechnology and agriculture. Biotechnol Adv 32:137–157

    Article  PubMed  Google Scholar 

  • Nam J, Matthysse AG, Gelvin SB (1997) Differences in susceptibility of Arabidopsis ecotypes to crown gall disease may result from a deficiency in T-DNA integration. Plant Cell 9:317–333

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Nigorikawa M, Watanabe A, Furukawa K, Sonoki T, Ito Y (2012) Enhanced saccharification of rice straw by overexpression of rice exo-glucanase. Rice 5:14

    Article  PubMed  PubMed Central  Google Scholar 

  • Nishimura A, Aichi I, Matsuoka M (2007) A protocol for Agrobacterium-mediated transformation in rice. Nat Protoc 1:2796–2802

    Article  Google Scholar 

  • Sato K, Yamane M, Yamaji N, Kanamori H, Tagiri A, Schwerdt JG, Fincher GB, Matsumoto T, Takeda K, Komatsuda T (2016) Alanine aminotransferase controls seed dormancy in barley. Nat Commun 7:11625

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Sparrow PAC, Townsend TM, Arthur A, Dale PJ, Irwin JA (2004) Genetic analysis of Agrobacterium tumefaciens susceptibility in Brassica oleracea. Theor Appl Genet 108:644–650

    Article  CAS  PubMed  Google Scholar 

  • The International Barley Sequencing Consortium (IBSC) (2012) A physical, genetic and functional sequence assembly of the barley genome. Nature 491:711–716

    Google Scholar 

  • Tingay S, McElroy D, Kalla R, Fieg S, Wang M, Thornton S, Brettell R (1997) Agrobacterium tumefaciens-mediated barley transformation. Plant J 11:1369–1376

    Article  CAS  Google Scholar 

  • Yeo FKS, Hensel G, Vozábová T, Martin-Sanz A, Marcel TC, Kumlehn J, Niks RE (2014) Golden SusPtrit: a genetically well transformable barley line for studies on the resistance to rust fungi. Theor Appl Genet 127:325–337

    Article  CAS  PubMed  Google Scholar 

  • Zhang X, Henriques R, Lin S-S, Niu Q-W, Chua N-H (2006) Agrobacterium-mediated transformation of Arabidopsis thaliana using the floral dip method. Nat Protoc 1:641–646

    Article  CAS  PubMed  Google Scholar 

Download references

Acknowledgements

We thank Tanya Filichkin and Laura Helgerson for their parts in developing the Oregon Promise population, Yuka Motoi (IPSR, Okayama Univ.) for technical assistance, and Dr. Yukihiro Ito (Tohoku Univ., Japan) for providing the pBUH3 vector. This work was supported by the Spanish Ministry of Science and Innovation (Project AGL2015-69435-C3-2-R) to L.C., the Gatsby Foundation and BBSRC Institutional Strategic Programme (BB/J004553/1) to M.M., and JSPS KAKENHI, Grant Numbers 24880025 and 16K18634 to H.H.

Author information

Authors and Affiliations

  1. Institute of Plant Science and Resources, Okayama University, 2-20-1 Chuo, Kurashiki, Japan

    Hiroshi Hisano & Kazuhiro Sato

  2. Department Crop and Soil Sciences, Washington State University, Mount Vernon, WA, USA

    Brigid Meints

  3. The Sainsbury Laboratory, Norwich Research Park, Norwich, UK

    Matthew J. Moscou

  4. Department Genetica y Produccion Vegetal, Estacion Experimental de Aula Dei, Zaragoza, Spain

    Luis Cistue & Begoña Echávarri

  5. Department Crop and Soil Science, Oregon State University, Corvallis, OR, USA

    Patrick M. Hayes

Authors
  1. Hiroshi Hisano
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Brigid Meints
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Matthew J. Moscou
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Luis Cistue
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Begoña Echávarri
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Kazuhiro Sato
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Patrick M. Hayes
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Hiroshi Hisano.

Ethics declarations

Conflict of interest

The authors declare that they have no conflict of interest.

Additional information

Communicated by Mark C. Jordan.

Electronic supplementary material

Below is the link to the electronic supplementary material.

Supplementary material 1 (DOCX 163 KB)

Supplementary material 2 (XLSX 520 KB)

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Hisano, H., Meints, B., Moscou, M.J. et al. Selection of transformation-efficient barley genotypes based on TFA (transformation amenability) haplotype and higher resolution mapping of the TFA loci. Plant Cell Rep 36, 611–620 (2017). https://doi.org/10.1007/s00299-017-2107-2

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00299-017-2107-2

Keywords

Search

Navigation