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Androgenesis, gynogenesis, and parthenogenesis haploids in cucurbit species

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Abstract

Haploids and doubled haploids are critical components of plant breeding. This review is focused on studies on haploids and double haploids inducted in cucurbits through in vitro pollination with irradiated pollen, unfertilized ovule/ovary culture, and anther/microspore culture during the last 30 years, as well as comprehensive analysis of the main factors of each process and comparison between chromosome doubling and ploidy identification methods, with special focus on the application of double haploids in plant breeding and genetics. This review identifies existing problems affecting the efficiency of androgenesis, gynogenesis, and parthenogenesis in cucurbit species. Donor plant genotypes and surrounding environments, developmental stages of explants, culture media, stress factors, and chromosome doubling and ploidy identification are compared at length and discussed as methodologies and protocols for androgenesis, gynogenesis, and parthenogenesis in haploid and double haploid production technologies.

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Abbreviations

2,4-D:

2,4-Dichlorophenoxyacetic acid

BAP:

6-Benzylaminopurine

CBM:

Cucumber basal medium

CMV:

Cucumber mosaic virus

CPA:

Chlorophenoxyacetic acid

DH:

Double haploid

DHL:

Double haploid line

ELISA:

Enzyme-linked immunosorbent assay

GA3 :

Gibberellic acid

IAA:

Indole-3-acetic acid

KT:

Kinetin

NAA:

α-Naphthaleneacetic acid

PGR:

Plant growth regulator

Put:

Putrescine

RAPD:

Random amplified polymorphic DNA

RFLP:

Restriction fragment length polymorphism

SNP:

Single nucleotide polymorphism

SSR:

Simple sequence repeats

TDZ:

Thidiazuron

WMV:

Watermelon mosaic virus

ZYMV:

Zucchini yellow mosaic virus

References

  • Abdollahi MR, Darbandi M, Hamidvand Y, Majdi M (2015) The influence of phytohormones, wheat ovary co-culture, and temperature stress on anther culture response of watermelon (Citrullus lanatus L.). Braz J Bot 38(3):447–456

  • Abdollahi MR, Najafi S, Sarikhani H, Moosavi SS (2016) Induction and development of anther-derived gametic embryos in cucumber (Cucumis sativus L.) by optimizing the macronutrient and agar concentrations in culture medium. Turk J Biol 40:1–10

    Article  Google Scholar 

  • Antos M, Bułat E, Zawiślak E (2001) Cucumber (Cucumis sativus L.) haploids induction with use of X-rays. Folia Hort 13(1A):81–84

  • Arafeh MO (2006) Production of double haploid squash plants. Cairo University, Cairo

    Google Scholar 

  • Ari E, Ikten H, Gocmen M, Coskun R, Eren A (2010) Comparative evaluation of different embryo rescue techniques on parthenogenetic melon (Cucumis melo L.) fruits induced with irradiated pollen. Afr J Biotechnol 9(33):5347–5356

    Google Scholar 

  • Bajaj YPS (1990) In vitro production of haploids and their use in cell genetics and plant breeding. In: Bajaj YPS (ed) Haploids in crop improvement I. Springer, Berlin, pp 3–44

    Chapter  Google Scholar 

  • Baktemur G, Taşkın H, Büyükalaca S (2013) Comparison of different methods for separation of haploid embryo induced through irradiated pollen and their economic analysis in melon (Cucumis melo var. inodorus). Sci World J 10:1–7

    Article  Google Scholar 

  • Baktemur G, Yücel NK, Taskın H, Çömlekçioğlu S, Büyükalaca S (2014) Effects of different genotypes and gamma ray doses on haploidization using irradiated pollen technique in squash. Turk J Biol 38(3):318–327

    Article  Google Scholar 

  • Bal U, Sari N, Yilmaz H (2003) Effects of E20 and Ms based media on in vitro induction of axillary buds and shoot development from haploid cucumis melon microcuttings. Pak J Biol Sci 6(13):1130–1138

    Article  Google Scholar 

  • Beharav A, Cohen Y (1995) Effect of kinetin and GA3 on in vitro ovule embryo culture of Cucumis melo L. Plant Growth Regul 16(3):267–269

    Article  CAS  Google Scholar 

  • Bögre L, Calderini O, Binarova P, Mattauch M, Till S, Kieger S, Jonak C, Pollaschek C, Barker P, Huskisson NS, Hirt H, Heberle-Borsa E (1999) A MAP kinase is activated late in plant mitosis and becomes localized to the plane of cell division. Plant Cell 11:101–113

    Article  PubMed  PubMed Central  Google Scholar 

  • Çağlar G, Abak K (1999a) In situ haploid embryo induction in cucumber (Cucumis sativus L.) after pollination by irradiated pollen. Turk J Agric For 23(EK1):63–72

  • Çağlar G, Abak K (1999b) Obtention of in vitro haploid plants from in situ induced haploid embryos in cucumber (Cucumis sativus L.). Turk J Agric For 23(3):283–290

  • Çağlar G, Abak K (1999c) Progress in the production of haploid embryos, plants and doubled haploids in cucumber (Cucumis sativus L.) by gamma irradiated pollen in Turkey. Acta Hort 492:317–322

    Article  Google Scholar 

  • Cecile D, Girard M, Lecoq H (2010) A novel natural mutation in HC-Pro responsible for mild symptomatology of Zucchini yellow mosaic virus (ZYMV, Potyvirus) in cucurbits. Arch Virol 155(3):397–401

    Article  CAS  Google Scholar 

  • Chambonnet D, Dumas De Vaulx R (1985) Obtention of embryos and plants from in vitro culture of unfertilized ovules of Cucurbita pepo. Cucurbit genetics. Coop Rep 8:66

    Google Scholar 

  • Chee RP, Leskovar DI, Cantliffe DJ (1992) Optimizing embryogenic callus and embryo growth of a synthetic seed system for sweet potato by varying media nutrient concentrations. J Am Soc Hortic Sci 117:663–667

    Google Scholar 

  • Chen JF, Cui L, Malik AA, Mbira KG (2011) In vitro haploid and dihaploid production via unfertilized ovule culture. Plant Cell Tissue Org Cult 104(3):311–319

    Article  Google Scholar 

  • Chu CC, Wang CC, Sun CS, Chen H, Yin KC, Chu CY, Bi FY (1975) Establishment of an efficient medium for anther culture of rice through comparative experiments on the nitrogen sources. J Sci China Math 18(5):659–668

    Google Scholar 

  • Claveria E, Garcia-Mas J, Dolcet-Sanjuan R (2005) Optimization of cucumber doubled haploid line production using in vitro rescue of in vivo induced parthenogenic embryos. J Am Soc Hort Sci 130(4):555–560

    Google Scholar 

  • Cuny F, Roudot AC (1991) Germination et croissance pollinique in vitro du pollen de melon (Cucumis melo L.) apres irradiations gamma. Environ Exp Bot 31(3):277–283

    Article  Google Scholar 

  • Cuny F, Dumas de Vaulx R, Longhi B, Siadous R (1992) Analyse des plantes de melon (Cucumis melo L.) issues de croisements avec du pollen irradie a differentes doses. Agronomie 12(8):623–630

  • Cuny F, Grotte M, Dumas de Vaulx R, Rieu A (1993) Effects of gamma irradiation of pollen on parthenogenetic haploid production in muskmelon (Cucumis melo L.). Environ Exp Bot 33(2):301–312

  • Damicone JP, Edelson JV, Sherwood JL, Myers LD, Motes JE (2007) Effects of border crops and intercrops on control of cucurbit virus diseases. Am Phytopathol Soc 91(5):509–516

    Google Scholar 

  • Davies PA, Morton S (1998) A comparison of barley isolated microspore and anther culture and the influence of cell culture density. Plant Cell Rep 17(3):206–210

    Article  CAS  Google Scholar 

  • Deunff EL, Sauton A (1994) Effect of parthenocarpy on ovule development in cucumber (Cucumis sativus L.) after pollination with normal and irradiated pollen. Sex Plant Reprod 7(4):221–228

    Article  Google Scholar 

  • Diao WP, Jia YY, Song H, Zhang XQ, Lou QF, Chen JF (2009) Efficient embryo induction in cucumber ovary culture and homozygous identification of the regenerants using SSR markers. Sci Hortic 119(3):246–251

    Article  CAS  Google Scholar 

  • Dolcet-Sanjuan R, Claveria E, Garcia-Mas J (2006) Cucumber (Cucumis sativus L.) dihaploid line production using in vitro rescue of in vivo induced parthenogenic embryos. Acta Hort 725(2):837–844

  • Dryanovska OA (1985) Induced callus in vitro from ovaries and anthers of species from the Cucurbitaceae family. C R Acad Bulg Sci 38:1243–1244

    Google Scholar 

  • Dryanovska OA, Ilieva IN (1983) In vitro anther and ovule cultures in muskmelon (Cucumis melo L.). Bulg Acad Sci 36:1107–1110

    Google Scholar 

  • Du SL, Wei HJ, Wei AM, Ma DH, Huo ZR (1999) Haploid plantlet regeneration of cucumber by pollination with irradiated pollens. Sci Agric Sin 32(2):107

    Google Scholar 

  • Dumas de Vaulx R (1979) Obtention de plantes haploides chez le melon (Cucumis melo L.) apres pollinisation par Cucumis ficifolius A. Rich C R Acad Sci III-Vie 289:875–878

    Google Scholar 

  • Dunwell JM (1976) A comparative study of environmental and developmental factors which influence embryo induction and growth in cultured anthers of Nicotiana tabacum. Environ Exp Bot 16(2–3):109–118

    Article  Google Scholar 

  • Dunwell JM (2010) Haploids in flowering plants: origins and exploitation. Plant Biotechnol J 8:377–424

    Article  CAS  PubMed  Google Scholar 

  • Falconer MM, Seagull RW (1987) Amiprophos-methyl (APM): a rapid, reversible, anti-microtubule agent for plant cell cultures. Protoplasma 136(2–3):118–124

    Article  CAS  Google Scholar 

  • Faris NM, Niemirowicz-Szczytt K (1999) Cucumber (Cucumis sativus L.) embryo development in situ after pollination with irradiated pollen. Acta Biol Cra Bot 41(1):111–118

  • Faris NM, Nikolova V, Niemirowicz-Szczytt K (1999) The effect of gamma irradiation dose on cucumber (Cucumis sativus L.) haploid embryo production. Acta Physiol Plant 21(4):391–396

    Article  Google Scholar 

  • Fattouh FA (2003) Double infection of a cucurbit host by zucchini yellow mosaic virus and cucumber mosaic virus. Plant Pathol J 2(2):85–90

    Article  Google Scholar 

  • Ferrie AMR, Caswell KL (2010) Isolated microspore culture techniques and recent progress for haploid and doubled haploid plant production. Plant Cell Tiss Org Cult 104(3):301–309

    Article  Google Scholar 

  • Ficcadenti N, Sestili S, Annibali S, Marco MD, Schiavi M (1999) In vitro gynogenesis to induce haploid plants in melon (Cucumis melo L.). J Genet Breed 53(3):255–257

  • Forster BP, Hebe rle-Bors E, Kasha KJ, Touraev A (2007) The resurgence of haploids in higher plants. Trends Plant Sci 12(8):368–375

    Article  CAS  PubMed  Google Scholar 

  • Gałązka J, Niemirowicz-Szczytt K (2013) Review of research on haploid production in cucumber and other cucurbits. Folia Hort 25(1):67–78

    Google Scholar 

  • Gamborg OL, Miller RA, Ojima K (1968) Nutrient requirements of suspension cultures of soybean root cells. Exp Cell Res 50:151–158

    Article  CAS  PubMed  Google Scholar 

  • Gémes-Juhász A, Jakše M (2005) Haploids in the improvement of miscellaneous crop species (Cucurbitaceae, Liliaceae, Asparagaceae, Chenopodiaceae, Araceae and Umbelliferae). In: Don Palmer CE, Keller WA, Kasha KJ (eds) Biotechnology in agriculture and forestry: haploids in crop improvement II. Springer, Berlin, pp 259–278

  • Gémes-Juhász A, Balogh P, Ferenczy A, Kristóf Z (2002) Effect of optimal stage of female gametophyte and heat treatment on in vitro gynogenesis induction in cucumber (Cucumis sativus L.). Plant Cell Rep 21(2):105–111

    Article  CAS  Google Scholar 

  • Gémesné-Juhász A, Venczel G, Balogh P (1997) Haploid plant induction in zucchini (Cucurbita pepo L. convar. giromontiina Duch) and in cucumber (Cucumis sativus L.) lines through in vitro gynogenesis. Acta Hort 447:623–625

    Article  Google Scholar 

  • Germanà MA (2011) Gametic embryogenesis and haploid technology as valuable support to plant breeding. Plant Cell Rep 30(5):839–857

    Article  PubMed  CAS  Google Scholar 

  • Godbole M, Murthy HN (2012a) In vitro production of haploids via parthenogenesis in culinary melon (cucumis melon var. acidulus). India. J Biotechnol 11(4):495–497

    CAS  Google Scholar 

  • Godbole M, Murthy HN (2012) Parthenogenetic haploid plants using gamma irradiated pollen in snapmelon (Cucumis melo var. momordica). Plant Cell Tiss Org Cult 109(1):167–170

  • Gonzalo MJ, Oliver M, Garcia-Mas J, Monfort A, Dolcet-Sanjuan R, Katzir N, Arús P, Monforte AJ (2005) Simple-sequence repeat markers used in merging linkage maps of melon (Cucumis melo L.). Theor Appl Genet 5(110):802–811

    Article  CAS  Google Scholar 

  • Gonzalo MJ, Claveria E, Monforte AJ, Dolcet-Sanjuan R (2011) Parthenogenic haploids in melon: generation and molecular characterization of a doubled haploid line population. J Am Soc Hort Sci 136(2):145–154

    CAS  Google Scholar 

  • Gürsöz N, Abak K, Pitrat M, Rode JC, Dumas de Vaulx R (1991) Obtention of haploid plants induced by irradiated pollen in watermelon (Citrillus lanatus L.). Cucurbit genetics. Coop Rep 4:109–110

    Google Scholar 

  • Hamidvand Y, Abdollahi MR, Chaichi M, Moosavi SS (2013) The effect of plant growth regulators on callogenesis and gametic embryogenesis from anther culture of cucumber (Cucumis sativus L.). Int J Agric Crop Sci 5(10):1089–1095

    Google Scholar 

  • Hansen NJP, Andersen SB (1995) In vitro chromosome doubling potential of colchicine, oryzalin, trifluralin, and APM in Brassica napus microspore culture. Euphytica 88(2):159–164

    Article  Google Scholar 

  • Kato A (2001) Heterofertilization exhibited by trifluralin-induced bicellular pollen on diploid and tetraploid maize crosses. Genome 44(6):1114–1121

    Article  CAS  PubMed  Google Scholar 

  • Katoh N, Hagimori M, Iwai S (1993) Production of haploid plants of melon by pseudofertilized ovule culture. Plant Tiss Cult Lett 10(1):60–66

    Article  CAS  Google Scholar 

  • Koksal N, Yetisir H, Sari N, Abak K (2002) Comparison of different in vivo methods for chromosome duplication in muskmelon (Cucumis melo). Acta Hort 588:293–298

    Article  CAS  Google Scholar 

  • Koli SP, Murthy HN (2013) Haploid plant regeneration from unpollinated ovules of cucumis melo L. var. Conomon cv. Mudicode. Br Biotechnol J 3(4):605–613

    Article  CAS  Google Scholar 

  • Košmrlj K, Murovec J, Bohanec B (2013) Haploid Induction in hull-less seed pumpkin through parthenogenesis induced by X-ray-irradiated pollen. J Am Soc Hort Sci 138(4):310–316

    Google Scholar 

  • Košmrlj K, Kastelec D, Bohanec B (2014) Styrian oil pumpkin pollen germinability at higher irradiation doses: optimization of the in vitro germination protocol and irradiation procedure. Turk J Biol 38(4):516–522

    Article  Google Scholar 

  • Kumar HGA, Murthy HN (2004) Effect of sugars and amino acids on androgenesis of Cucumis sativus. Plant Cell Tiss Org Cult 78(3):201–208

    Article  CAS  Google Scholar 

  • Kumar HGA, Murthy HN, Paek KY (2003) Embryogenesis and plant regeneration from anther cultures of Cucumis sativus L. Sci Hortic 98(3):213–222

    Article  CAS  Google Scholar 

  • Kumar HGA, Ravishankar BV, Murthy HN (2004) The Influence of Polyamines on Androgenesis of Cucumis sativus L. Eur J Hortic Sci 69(5):201–205

    CAS  Google Scholar 

  • Kurtar ES, Balkaya A (2010) Production of in vitro haploid plants from in situ induced haploid embryos in winter squash (Cucurbita maxima Duchesne ex Lam.) via irradiated pollen. Plant Cell Tissue Org Cult 102(3):267–277

  • Kurtar ES, Sari N, Abak K (2002) Obtention of haploid embryos and plants through irradiated pollen technique in squash (Cucurbita pepo L.). Euphytica 127(3):335–344

    Article  CAS  Google Scholar 

  • Kurtar ES, Balkaya A, Ozbakir M, Ofluoglu T (2009) Induction of haploid embryo and plant regeneration via irradiated pollen technique in pumpkin (Cucurbita moschata Duchesne ex. Poir). Afr J Biotechnol 8(21):5944–5951

    Article  Google Scholar 

  • Kuzuya M, Hosoya K, Yashiro K, Tomita K, Ezura H (2003) Powdery mildew (Sphaerotheca fuliginea) resistance in melon is selectable at the haploid level. J Exp Bot 54(384):1069–1074

    Article  CAS  PubMed  Google Scholar 

  • Kwack SN, Fujieda K (1988) Somatic embryogenesis in cultured unfertilized ovules of Cucurbita moschata. J Jpn Soc Hortic Sci 57(1):34–42

    Article  Google Scholar 

  • Lazarte JE, Sasser CC (1982) Asexual embryogenesis and plantlet development in anther culture of Cucumis sativus L. HortScience 17:88

    Google Scholar 

  • Lei C, Chen JF, Qian CT, Zhang YB, Song H (2004) Recovery of cucumber (Cucumis sativus L.) haploid plants through pollination by irradiated pollens and embryo culture. Acta Bot Boreali-Occident Sin 24(9):1739–1743

  • Lei C, Chen JF, Qian CT, Zhang XQ, Zhang YB (2006) Studies on induction of haploid cucumbers by irradiated pollen pollination and their characterization. Sci Agric Sin 39(7):1428–1436

    Google Scholar 

  • Li JW, Si SW, Cheng JY, Li JX, Liu JQ (2013) Thidiazuron and silver nitrate enhanced gynogenesis of unfertilized ovule cultures of Cucumis sativus. Biol Plant 57(1):164–168

    Article  CAS  Google Scholar 

  • Lichter R (1982) Induction of haploid plants from isolated pollen of Brassica napus. Z Pflanzenphysiol 105:427–434

    Article  Google Scholar 

  • Lim W, Earle ED (2008) Effect of in vitro and in vivo colchicine treatments on pollen production and fruit recovery on melon plants obtained after pollination with irradiated pollen. Plant Cell Tiss Org Cult 95(1):115–124

    Article  CAS  Google Scholar 

  • Lim W, Earle ED (2009) Enhanced recovery of doubled haploid lines from parthenogenetic plants of melon (Cucumis melo L.). Plant Cell Tiss Org Cult 98:351–356

    Article  Google Scholar 

  • Lofti M, Salehi S (2008) Detection of cucumber parthenogenic haploid embryos by floating the immature seeds in liquid medium. In: Pitrat M (ed) Proceeding of IXth EUCARPIA meeting on genetics and breeding of Cucurbitaceae. Avignon, France, pp 375–380

    Google Scholar 

  • Lofti M, Kashi A, Onsinejad R (1999) Induction of parthenogenetic embryos by irradiated pollen in cucumber. Acta Hort. 492:323–328

    Google Scholar 

  • Lofti M, Alan AR, Henning MJ, Jahn MM, Earle ED (2003) Production of haploid and double haploid plants of melon (Cucumis melo L.) for use in breeding for multiple virus resistance. Plant Cell Rep 21(11):1121–1128

    Article  CAS  Google Scholar 

  • Malik AA, Cui L, Zhang SX, Chen JF (2011) Efficiency of SSR makers for determining the origin of melon plantlets derived through unfertilized ovary culture. Hort Sci 38(1):27–34

    CAS  Google Scholar 

  • Maluszynski M, Kasha KJ, Forster BP, Szarejko I (2003) Doubled haploid production in crop plants: a manual. Kluwer Academic, London

    Book  Google Scholar 

  • Maraschin SF, Priester WD, Spaink HP, Wang M (2005) Androgenic switch: an example of plant embryogenesis from the male gametophyte perspective. J Exp Bot 56(417):1711–1726

    Article  CAS  PubMed  Google Scholar 

  • Masuda K, Kikuta Y, Okazawa YA (1981) A revision of the medium for somatic embryogenesis in carrot suspension culture. J Fac Agr Hokkaido Univ 60(3):183–193

    CAS  Google Scholar 

  • Mcgrath MT (2001) Fungicide resistance in cucurbit powdery mildew: experiences and challenges. Plant Dis 85(3):236–245

    Article  Google Scholar 

  • Metwally EI, Moustafa SA, EI-Sawy BI, Shalaby TA (1998a) Haploid plantlets derived by anther culture of Cucurbita pepo. Plant Cell Tiss Org Cult 52(3):171–176

    Article  CAS  Google Scholar 

  • Metwally EI, Moustafa SA, El-Sawy BI, Haroun SA, Shalaby TA (1998b) Production of haploid plants from in vitro culture of unpollinated ovules of Cucurbita pepo L. Plant Cell Tiss Org Cult 52(3):117–121

    Article  CAS  Google Scholar 

  • Min ZY, Li H, Zou T, Tong L, Cheng J, Sun XW (2016) Studies of in vitro culture and plant regeneration of unfertilized ovary of pumpkin. Chin Bull Bot 51(1):74–80

    Google Scholar 

  • Mohamed MF, Refaei EFS (2004) Enhanced haploids regeneration in anther culture of summer squash (Cucurbita pepo L.). Cucurbit genetics. Coop Rep 27:57–60

    Google Scholar 

  • Moqbeli E, Peyvast G, Hamidoghli Y, Olfati JA (2013) In vitro cucumber haploid line generation in several new cultivars. AsPac J Mol Biol Biotechnol 21(1):18–25

    Google Scholar 

  • Moussa H, Salem A (2009) Induction of parthenocarpy in watermelon (Citrullus lanatus) cultivars by gamma irradiation. Acta Agron Hung 57(2):137–148

    Article  CAS  Google Scholar 

  • Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15(3):473–497

    Article  CAS  Google Scholar 

  • Nasertorabi M, Madadkhah E, Moghbeli H, Roain A, Moghbeli E (2012) Enhanced production of doubled haploid lines from parthenogenetic Iranian melon plants obtained of irradiated pollen (Cucumis melo L.). Int Res J Appl Basic Sci 3(8):1595–1600

  • Niemirowicz-Szczytt K, Faris NM, Nikolova V, Rakoczy-Troja nowska M, Malepszy S (1995) Optimization of cucumber (Cucumis sativus L.) haploid production and doubling. In: Lester (ed) Proceeding of Cucurbitaceae ’94, pp 169–171

  • Palti J, Cohen Y (1980) Downy mildew of cucurbits (Pseudoperonospora cubensis): the fungus and its hosts, distribution, epidemiology and control. Phytoparasitica 8(2):109–147

    Article  Google Scholar 

  • Perez-Garcia A, Romero DD, Lopez-Ruiz F, De VA, Tores JA (2009) The powdery mildew fungus Podosphaera fusca (synonym Podosphaera xanthii), a constant threat to cucurbits. Mol Plant Pathol 10(2):153–160

    Article  PubMed  Google Scholar 

  • Plapung P, Khamsukdee S, Potapohn N, Smitamana P (2014a) Screening for cucumber mosaic resistant lines from the ovule culture derived double haploid cucumbers. Am J Agric Biol Sci 9(3):261–269

    Article  Google Scholar 

  • Plapung P, Khumsukdee S, Smitamana P (2014b) Development of cucumber lines resistant to Cucumber mosaic virus by ovule culture. Int J Agric Technol 10(3):733–741

    Google Scholar 

  • Przyborowski JA (1996) Haploidy in cucumber (Cucumis sativus L.). In: Jain SM, Sopory SK, Veilleux RE (eds) In vitro haploid production in higher plants: volume 3—Important selected plants. Kluwer Academic, Dordrecht, pp 91–98

    Chapter  Google Scholar 

  • Przyborowski JA, Niemirowicz-Szczytt K (1994) Main factors affecting cucumber (Cucumis sativus L.) haploid embryo development and haploid plant characteristics. Plant Breed 112(1):70–75

    Article  Google Scholar 

  • Rakha MT, Metwally EI, Moustafa SA, Etman AA, Dewir YH (2012) Evaluation of regenerated strains from six Cucurbita interspecific hybrids obtained through anther and ovule in vitro cultures. Aust J Crop Sci 6(1):23–30

    CAS  Google Scholar 

  • Roberts-Oehlschlager SL, Dunwell JM (1990) Barley anther culture: pretreatment on mannitol stimulates production of microspore-derived embryos. Plant Cell Tiss Org Cult 20(3):235–240

    CAS  Google Scholar 

  • Sari N, Abak K (1996) Effect of colchicine treatment with different doses and periods on in vitro chromosome duplication in haploid watermelon. Turk J Agric For 20(6):555–559

    CAS  Google Scholar 

  • Sari N, Yetişir H (2002) Some agronomical characteristics of doubled haploid lines produced by irradiated pollen technique and parental diploid genotypes in melons. Turk J Agric For 26:311–317

    Google Scholar 

  • Sari N, Abak K, Pitrat M, Rode JC, Dumas de Vaulx R (1994) Induction of parthenogenetic haploid embryos after pollination by irradiated pollen in watermelon. HortScience 29(10):1189–1190

    Google Scholar 

  • Sari N, Abak K, Pitrat M (1999) Comparison of ploidy level screening methods in watermelon: Citrullus lanatus (Thunb.) Matsum. and Nakai. Sci Hortic 82(3):265–277

  • Sari N, Solmaz I, Ekiz H, Yetisir H, Yucel S (2010a) New fusarium wilt resistant melon (Cucumis melo var. cantalupensis) varieties developed by dihaploidization: Sari F1, Yetisir F1, Solmaz F1, Emin F1 and Yucel F1. Acta Hort 871:267–271

    Article  Google Scholar 

  • Sari N, Solmaz I, Kasapoglu S, Gursoy I, Szamosi C, Unlu H, Park KS (2010b) Effect of different pollination dates with irradiated pollens on fruit set, haploid embryo induction and plant obtention in Turkish (Kirkagac, Yuva and Hasanbey) melons. Acta Hort 871(1):639–648

    Article  Google Scholar 

  • Sauton A (1988) Effect of season and genotype on gynogenetic haploid production in muskemlon, Cucumis melo L. Sci Hortic 35(88):71–75

    Article  Google Scholar 

  • Sauton A (1989) Haploid gynogenesis in Cucumis sativus induced by irradiated pollen. Cucurbit genetics. Coop Rep 12:22–23

    Google Scholar 

  • Sauton A, Dumas de Vaulx R (1987) Production of haploid plants in melon (Cucumis melo L.) as a result of gynogenesis induced by irradiated pollen. Agronomie 7(2):141–147

  • Sauton A, Dumas de Vaulx R (1988) Doubled haploid production in melon (Cucumis melo L.). In: Proceedings of eucarpia meeting on cucurbit genetics and breeding. Avignon Monfavet, France, pp 119–128

  • Savin F, Decomble V, Le Couvioir M, Hallard J (1988) The X-ray detection of haploid embryos arisen in muskmelon (Cucumis melo L.) seeds and resulting from a parthenogenetic development induced by irradiated pollen. Cucurbit Genet Coop Rep 11:39–42

    Google Scholar 

  • Sestili S, Ficcadenti N (1996) Irradiated pollen for haploid production. In: Jain JM, Sopory SK, Veilleux RE (eds) In vitro haploid production in higher plants: oil, ornamental and miscellaneous plants. Kluwer Academic, Dordrecht, pp 263–274

    Google Scholar 

  • Shail JW, Robinson RW (1987) Anther and ovule culture of Cucurbita. Cucurbit Genet Coop Rep 10:92

    Google Scholar 

  • Shalaby TA (2006) Embryogenesis and plantlets regeneration from anther culture of squash plants (Cucurbita pepo L.) as affected by different genotypes. J Agric Res Tanta Univ 32(1):173–183

  • Shalaby TA (2007) Factors affecting haploid induction through in vitro gynogenesis in summer squash (Cucurbita pepo L.). Sci Hortic 115(1):1–6

  • Siebel J, Pauls KP (1989) A comparison of anther and microspore culture as a breeding tool in Brassica napus. Theor Appl Genet 78(4):473–479

    Article  CAS  PubMed  Google Scholar 

  • Śmiech M, Sztangret-Wiśniewska J, Galecka T, Korzeniewska A, Marzec L, Kolakowska G, Piskurewicz U, Niemirowicz-Szczytt K (2008) Potential use of RAPD markers in characteristics of cucumber (Cucumis sativus L.) haploids and double-haploids. Acta Soc Bot Pol 77(1):29–34

  • Solmaz İ, Sarı N, Gürsoy I, Kasapoğlu S (2011) Comparison of in vivo and in vitro colchicine application for production of dihaploid ‘Kirkagac’ and ‘Yuva Hasanbey’ melons. Afr J Biotechnol 10(70):15717–15724

    Article  CAS  Google Scholar 

  • Song H, Lou QF, Luo XD, Wolukau JN, Diao WP, Qian CT, Chen JF (2007) Regeneration of doubled haploid plants by androgenesis of cucumber (Cucumis sativus L.). Plant Cell Tiss Org Cult 90(3):245–254

  • Sun SR, Zhai QH, Hu JB, Chen JF, Zhang P (2009) Effects of several physiological factors on embryo formation in unpollinated ovary culture of pumpkin (Cucurbita moschata Duch. ex Poiret). Plant Physiol Commun 45(10):977–980

  • Suprunova T, Shmykova N (2008) In vitro induction of haploid plants in unpollinated ovules, anther and microspore culture of Cucumis sativus. In: Pitra t M (ed) Proceedings of the IXth EUCARPIA meeting on genetics and breeding of Cucurbitaceae. Avignon, France, pp 371–374

  • Swaminathan MS, Singh MP (1958) X-ray induced somatic haploidy in watermelon. Curr Sci 27:63–64

    Google Scholar 

  • Sztangret-Wisniewska J, Galecka T, Korzeniewska A, Marzec L, Kolakowska G, Piskurewicz U, Śmiech M, Niemirowicz-Szczytt K (2006) Characteristics of double-haploid cucumber (Cucumis sativus L.) lines resistant to downy mildew (Pseudoperonospora cubensis [Berk. et Curt.] Rostovzev). Cucurbitaceae 515–526

  • Tantasawat PA, Sorntip A, Poolsawat O, Chaowiset W, Pornbungkerd P (2015) Evaluation of factors affecting embryo-like structure and callus formation in unpollinated ovary culture of cucumber (Cucumis sativus). Int J Agric Biol 17(3):613–618

    Article  CAS  Google Scholar 

  • Taşkın H, Yücel NK, Baktemur G, Çömlekçioğlu S, Büyükalaca S (2013) Effects of different genotypes and gamma ray doses on haploidization with irradiated pollen technique in watermelon (Citrullus lanatus L.). Can J Plant Sci 93(6):1165–1168

    Article  Google Scholar 

  • Touraev A, Pfosser M, Vicente O, Heberle-Bors E (1996) Stress as the major signal controlling the developmental fate of tobacco microspores: towards a unified model of induction of microspore/pollen embryogenesis. Planta 200(1):144–152

    Article  CAS  Google Scholar 

  • Truong-Andre I (1988) In vitro haploid plants derived from pollination by irradiated pollen of cucumber. Proceedings of eucarpia meeting on cucurbit genetics and breeding. Avignon Monfavet, France, pp 143–144

    Google Scholar 

  • Wan Y, Duncan DR, Rayburn AL, Petolino JF, Widholm JM (1991) The use of antimicrotubule herbicides for the production of doubled haploid plants from anther-derived maize callus. Theor Appl Genet 81(2):205–211

    Article  CAS  PubMed  Google Scholar 

  • Xie M, Qin LY, Pan JS, He HL, Wu AZ, Cai R (2005) Flower morphogenesis and microspore development versus anther culture of cucumber. Acta Bot Boreali-Occident Sin 25(6):1096–1100

    Google Scholar 

  • Xie B, Wang XF, Fan ZC (2006) Improved conditions of in vitro culture of unpollinated ovules and production of embryonary sac plants in summer squash (Cucurbita pepo L.). Sci Agric Sin 39(1):132–138

  • Xue GR, Yu WY, Fei KW, Cui HN, Sun RX (1983) Watermelon plants derived by in vitro anther culture. Plant Physiol Commun 4:40–42

    Google Scholar 

  • Xue GR, Yu WY, Yang ZY, Sun RY (1988) The induction of watermelon pollen plants and a preliminary observation of the posterity. Hereditas 10(2):5–8

    Google Scholar 

  • Yashiro K, Hosoya K, Kuzuya M, Tomita K (2002) Efficient production of doubled haploid melon plants by modified colchicine treatment of parthenogenetic haploids. Acta Hort 588:335–338

    Article  Google Scholar 

  • Yetisir H, Sari N (2003) A new method for haploid muskmelon (Cucumis melo L.) dihaploidization. Sci Hortic 98:277–283

    Article  Google Scholar 

  • Zaki M, Dickinson H (1995) Modification of cell development in vitro: the effect of colchicine on anther and isolated microspore culture in Brassica napus. Plant Cell Tiss Org Cult 40(3):255–270

    Article  CAS  Google Scholar 

  • Zhan Y, Chen JF, Malik AA (2009) Embryoid induction and plant regeneration of cucumber (Cucumis sativus L.) through microspore culture. Acta Hortic Sin 36(2):221–226

  • Zhang YB, Chen JF, Yi HP, Lei C, Wu MZ (2006) Induction of haploid melon (Cucumis melo) plants by pollination with irradiated pollens. J Fruit Sci 23(6):892–895

    Google Scholar 

  • Zhao J, Simmonds DH (1995) Application of trifluralin to embryogenic microspore cultures to generate doubled haploid plants in Brassica napus. Physiol Plant 95:304–309

    Article  CAS  Google Scholar 

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Acknowledgments

This work was supported by Natural Science Foundation of Henan Province (CN) (162102110099).

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Authors and Affiliations

  1. Department of Vegetable Breeding and Biotechnology, Xinxiang Academy of Agricultural Sciences, No. 518, Xiner Road, Xinxiang, Henan, China

    Yan-Qi Dong, Xi-Cun Liu, Jin-Hua Huang, Wen-Ying Wang & Zhen-Hai Tang

  2. Institute of Horticulture, Henan Academy of Agricultural Sciences, No. 116, Huayuan Road, Zhengzhou, China

    Wei-Xing Zhao, Xiao-Hui Li, Ning-Ning Gao & Xiao-Li Xu

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  1. Yan-Qi Dong
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Communicated by N. Stewart.

Wei-Xing Zhao is a co-first author.

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Dong, YQ., Zhao, WX., Li, XH. et al. Androgenesis, gynogenesis, and parthenogenesis haploids in cucurbit species. Plant Cell Rep 35, 1991–2019 (2016). https://doi.org/10.1007/s00299-016-2018-7

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