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Topoisomerase II-associated protein PAT1H1 is involved in the root stem cell niche maintenance in Arabidopsis thaliana

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PAT1H1, one of the homologues of Topoisomerase II-associated protein, is involved in the maintenance of root stem cell niche through the interaction with NINJA.

Abstract

The root stem cell niche, which possesses four mitotically inactive quiescent cells (QC) and the surrounding mitotically active stem cells, is critical for root development in Arabidopsis thaliana. However, the molecular regulation of the maintenance of root stem cell niche identity is still not fully understood. Here we show that one of the homologues of Topoisomerase II-associated protein, here named as PAT1H1, could regulate root stem cell niche identity. The pat1h1 mutant showed higher frequency of QC cell division and root distal stem cell (DSC) differentiation. With a high expression in roots, PAT1H1 was found to interact with the jasmonic acid (JA) signalling negative regulator Novel Interactor of JAZ (NINJA) and thus regulate root DSC niche identity. Consistent with the active QC cell division, which rarely occurs in wild-type controls, the pat1h1 mutant displayed higher expression of CYCB1 in the root stem cell niche. Together our data reveals that PAT1H1 maintains root stem cell niche stability through the interaction with NINJA and the regulation of cell division.

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Acknowledgments

H.T. is supported by the National Natural Science Foundation of China (project 31500229) and Shandong Provincial Natural Science Foundation, China (project ZR2013CQ042). Z.D. is funded by Grants from the National Basic Research Program of China (Grant No. 2015CB942900), the National Natural Science Foundation of China (Projects 31470371). The authors acknowledge B. Scheres, T. Laux, C.-Y. Li, P. Benfey and E. Farmer for published materials.

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Authors and Affiliations

  1. The Key Laboratory of Plant Cell Engineering and Germplasm Innovation, Ministry of Education, School of Life Sciences, Shandong University, Jinan, 250100, People’s Republic of China

    Qianqian Yu, Jiajia Liu, Huihui Zheng, Yuebin Jia, Huiyu Tian & Zhaojun Ding

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  1. Qianqian Yu
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  2. Jiajia Liu
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Correspondence to Huiyu Tian.

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Communicated by K. Chong.

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Yu, Q., Liu, J., Zheng, H. et al. Topoisomerase II-associated protein PAT1H1 is involved in the root stem cell niche maintenance in Arabidopsis thaliana . Plant Cell Rep 35, 1297–1307 (2016). https://doi.org/10.1007/s00299-016-1961-7

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