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Dendrobium micropropagation: a review

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Abstract

Dendrobium is one of the largest and most important (ornamentally and medicinally) orchid genera. Tissue culture is now an established method for the effective propagation of members of this genus. This review provides a detailed overview of the Dendrobium micropropagation literature. Through a chronological analysis, aspects such as explant, basal medium, plant growth regulators, culture conditions and final organogenic outcome are chronicled in detail. This review will allow Dendrobium specialists to use the information that has been documented to establish, more efficiently, protocols for their own germplasm and to improve in vitro culture conditions based on the optimized parameters detailed in this review. Not only will this expand the use for mass propagation, but will also allow for the conservation of important germplasm. Information on the in vitro responses of Dendrobium for developing efficient protocols for breeding techniques based on tissue culture, such as polyploidization, somatic hybridization, isolation of mutants and somaclonal variants and for synthetic seed and bioreactor technology, or for genetic transformation, is discussed in this review. This is the first such review on this genus and represents half a decade of literature dedicated to Dendrobium micropropagation.

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Authors and Affiliations

  1. P. O. Box 7, Miki-cho Post Office, Ikenobe 3011-2, Kagawa, 761-0799, Japan

    Jaime A. Teixeira da Silva

  2. Department of Rural Development, Centro de Ciências Agrárias, UFSCar, Via Anhanguera, km 174, CP 153, CEP 13.600-970, Araras, Brazil

    Jean Carlos Cardoso

  3. Research Institute of Nyíregyháza, University of Debrecen, Nyíregyháza, P.O. Box 12, 4400, Debrecen, Hungary

    Judit Dobránszki

  4. Key Laboratory of South China Agricultural Plant Molecular Analysis and Gene Improvement, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou, 510650, China

    Songjun Zeng

Authors
  1. Jaime A. Teixeira da Silva
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  2. Jean Carlos Cardoso
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  3. Judit Dobránszki
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  4. Songjun Zeng
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Correspondence to Jaime A. Teixeira da Silva, Jean Carlos Cardoso, Judit Dobránszki or Songjun Zeng.

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Communicated by N. Stewart.

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Teixeira da Silva, J.A., Cardoso, J.C., Dobránszki, J. et al. Dendrobium micropropagation: a review. Plant Cell Rep 34, 671–704 (2015). https://doi.org/10.1007/s00299-015-1754-4

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  • DOI: https://doi.org/10.1007/s00299-015-1754-4

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