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Effects of cryopreservation of Phaseolus vulgaris L. seeds on early stages of germination

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Abstract

In this work, we studied the effects of cryopreservation on various parameters of early stages of germination of Phaseolus vulgaris seeds (0, 7 and 14 days). Percentages of germination, fresh mass of different plant parts, levels of chlorophyll pigments (a, b, total), malondialdehyde, other aldehydes, phenolics (cell wall-linked, free, and total) and protein were determined. No phenotypic changes were observed visually in seedlings recovered from cryopreserved seeds. However, several significant effects of seed liquid nitrogen exposure were recorded at the biochemical level. There was a significant negative effect of cryopreservation on shoot protein content, which decreased from 3.11 mg g−1 fresh weight for non-cryopreserved controls to 0.44 mg g−1 fresh shoot weight for cryopreserved seeds. On the other hand, cryopreservation significantly increased levels of other aldehydes than malondialdehyde in shoots at day 7, from 56.47 μmol g−1 for non-cryopreserved controls to 253.19 μmol g−1 fresh shoot weight for cryopreserved samples. Liquid nitrogen exposure significantly reduced phenolics contents (free, cell-wall linked, total) in roots at day 7 after onset of germination. In general, roots were more affected by cryostorage compared with other plant parts, while leaves were the least affected. The effects of seed cryopreservation seem to decline progressively along with seedling growth.

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Acknowledgments

This research was supported by the Cuban Ministry for Superior Education. We are grateful to Mr. Roberto Méndez, Mrs. Maribel Rivas, Mr. René Carlos Rodríguez, Mr. Dariel López, Mr. Félix Palau, Mrs. Lourdes Yabor, Mrs. Bárbara Valle, Mrs. Julia Martínez, Mrs. Alitza Iglesias, and Mr. Yosvany Palmero for their excellent technical assistance and important experimental suggestions.

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Authors and Affiliations

  1. Faculty of Agronomy, Universidad de Ciego de Ávila, Ciego de Ávila, 69450, Cuba

    Inaudis Cejas, Karel Vives & Taletha Laudat

  2. Laboratory for Plant Breeding, Centro de Bioplantas, Universidad de Ciego de Ávila, Ciego de Ávila, 69450, Cuba

    Justo González-Olmedo, Marcos Edel Martínez-Montero & José Carlos Lorenzo

  3. IRD, UMR DIADE, 911 Avenue Agropolis, BP 64501, 34394, Montpellier Cedex 5, France

    Florent Engelmann

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  1. Inaudis Cejas
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  2. Karel Vives
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  3. Taletha Laudat
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Correspondence to José Carlos Lorenzo.

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Communicated by S. Merkle.

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Cejas, I., Vives, K., Laudat, T. et al. Effects of cryopreservation of Phaseolus vulgaris L. seeds on early stages of germination. Plant Cell Rep 31, 2065–2073 (2012). https://doi.org/10.1007/s00299-012-1317-x

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