Abstract
Tree peony (Paeonia suffruticosa Andrews), a woody deciduous shrub, belongs to the section Moutan DC. in the genus of Paeonia of the Paeoniaceae family. To increase the efficiency of breeding, two EST-derived marker systems were developed based on a tree peony expressed sequence tag (EST) database. Using target region amplification polymorphism (TRAP), 19 of 39 primer pairs showed good amplification for 56 accessions with amplicons ranging from 120 to 3,000 bp long, among which 99.3% were polymorphic. In contrast, 7 of 21 primer pairs demonstrated adequate amplification with clear bands for simple sequence repeats (SSRs) developed from ESTs, and a total of 33 alleles were found in 56 accessions. The similarity matrices generated by TRAP and EST-SSR markers were compared, and the Mantel test (r = 0.57778, P = 0.0020) showed a moderate correlation between the two types of molecular markers. TRAP markers were suitable for DNA fingerprinting and EST-SSR markers were more appropriate for discriminating synonyms (the same cultivars with different names due to limited information exchanged among different geographic areas). The two sets of EST-derived markers will be used further for genetic linkage map construction and quantitative trait locus detection in tree peony.
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Acknowledgments
This work was funded by the National High Technology Research and Development Program of China (863 Program, grant number 2006AA100109), the Pilot Research Program of Institute of Botany, the Chinese Academy of Sciences (2005-2008), National Natural Science Foundation of China (Grant No. 30800760 and No. 30571306), the Scientific Research Foundation for Returned Overseas Chinese Scholars from Ministry of Education of China ((2010) 1561), and the Knowledge Innovation Program of the Chinese Academy of Sciences (KSCX2-YW-Z-064). The authors thank Professor Yingqing Lu of State Key Laboratory of Systematic and Evolutionary Botany, Institute of Botany, the Chinese Academy of Sciences, for her valuable suggestions. The authors thank Tao Xia from Nanjing Forestry University for his technical assistance. The authors are also thankful to Dr. Jan De Riek, from Institute for Agriculture and Fishery Sciences, Belgium and Carl Ng from College Dublin (Ireland), for the manuscript revision.
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Zhang, J.J., Shu, Q.Y., Liu, Z.A. et al. Two EST-derived marker systems for cultivar identification in tree peony. Plant Cell Rep 31, 299–310 (2012). https://doi.org/10.1007/s00299-011-1164-1
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DOI: https://doi.org/10.1007/s00299-011-1164-1