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Analysis of genomic DNA of DcACS1, a 1-aminocyclopropane-1-carboxylate synthase gene, expressed in senescing petals of carnation (Dianthus caryophyllus) and its orthologous genes in D. superbus var. longicalycinus

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Abstract

Carnation (Dianthus caryophyllus) flowers exhibit climacteric ethylene production followed by petal wilting, a senescence symptom. DcACS1, which encodes 1-aminocyclopropane-1-carboxylate synthase (ACS), is a gene involved in this phenomenon. We determined the genomic DNA structure of DcACS1 by genomic PCR. In the genome of ‘Light Pink Barbara’, we found two distinct nucleotide sequences: one corresponding to the gene previously shown as DcACS1, designated here as DcACS1a, and the other novel one designated as DcACS1b. It was revealed that both DcACS1a and DcACS1b have five exons and four introns. These two genes had almost identical nucleotide sequences in exons, but not in some introns and 3′-UTR. Analysis of transcript accumulation revealed that DcACS1b is expressed in senescing petals as well as DcACS1a. Genomic PCR analysis of 32 carnation cultivars showed that most cultivars have only DcACS1a and some have both DcACS1a and DcACS1b. Moreover, we found two DcACS1 orthologous genes with different nucleotide sequences from D. superbus var. longicalycinus, and designated them as DsuACS1a and DsuACS1b. Petals of D. superbus var. longicalycinus produced ethylene in response to exogenous ethylene, accompanying accumulation of DsuACS1 transcripts. These data suggest that climacteric ethylene production in flowers was genetically established before the cultivation of carnation.

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Acknowledgments

This study was supported in part by a Grant-in-Aid (19380024 to S.S.) for Scientific Research from the Japan Society for the Promotion of Science.

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Authors and Affiliations

  1. Laboratory of Genetic Engineering, Graduate School of Life and Environmental Sciences, Kyoto Prefectural University, Kyoto, 606-8522, Japan

    Taro Harada, Yuka Torii, Shigeto Morita, Takehiro Masumura & Shigeru Satoh

  2. Laboratory of Genetic Engineering, Faculty of Agriculture, Kyoto Prefectural University, Kyoto, 606-8522, Japan

    Yuino Murakoshi

  3. National Institute of Floricultural Sciences, National Agriculture and Food Research Organization, Tsukuba, Ibaraki Prefecture, 305-8519, Japan

    Koji Tanase & Takashi Onozaki

  4. Kyoto Prefectural Institute of Agricultural Biotechnology, Seika-cho, Kyoto Prefecture, 619-0224, Japan

    Shigeto Morita, Takehiro Masumura & Shigeru Satoh

Authors
  1. Taro Harada
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  2. Yuino Murakoshi
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  3. Yuka Torii
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  4. Koji Tanase
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  5. Takashi Onozaki
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  6. Shigeto Morita
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  7. Takehiro Masumura
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  8. Shigeru Satoh
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Corresponding author

Correspondence to Shigeru Satoh.

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Communicated by P. Puigdomenech.

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Harada, T., Murakoshi, Y., Torii, Y. et al. Analysis of genomic DNA of DcACS1, a 1-aminocyclopropane-1-carboxylate synthase gene, expressed in senescing petals of carnation (Dianthus caryophyllus) and its orthologous genes in D. superbus var. longicalycinus . Plant Cell Rep 30, 519–527 (2011). https://doi.org/10.1007/s00299-010-0962-1

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  • DOI: https://doi.org/10.1007/s00299-010-0962-1

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