Abstract
Transgenic hairy root system is important in several recalcitrant plants, where Agrobacterium tumefaciens-mediated plant transformation and generation of transgenic plants are problematic. Jute (Corchorus spp.), the major fibre crop in Indian subcontinent, is one of those recalcitrant plants where in vitro tissue culture has provided a little success, and hence, Agrobacterium-mediated genetic transformation remains to be a challenging proposition in this crop. In the present work, a system of transgenic hairy roots in Corchorus capsularis L. has been developed through genetic transformation by Agrobacterium rhizogenes harbouring two plasmids, i.e. the natural Ri plasmid and a recombinant binary vector derived from the disarmed Ti plasmid of A. tumefaciens. Our findings indicate that the system is relatively easy to establish and reproducible. Molecular analysis of the independent lines of transgenic hairy roots revealed the transfer of relevant transgenes from both the T-DNA parts into the plant genome, indicating the co-transformation nature of the event. High level expression and activity of the gusA reporter gene advocate that the transgenic hairy root system, thus developed, could be applicable as gene expression system in general and for root functional genomics in particular. Furthermore, these transgenic hairy roots can be used in future as explants for plantlet regeneration to obtain stable transgenic jute plants.
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Acknowledgments
The authors thank Prof. Paul J. J. Hooykaas for granting permission to use the Agrobacterium rhizogenes LBA1334 strain. We also thank Prof. S. K. Sen and Dr. A. Basu for their cooperation and help. Financial assistance in the form of research scholarship to T.C. from IIT-Kharagpur, and Grant supports from the Department of Biotechnology and Department of Science & Technology, Government of India are acknowledged.
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Communicated by P. Kumar.
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Chattopadhyay, T., Roy, S., Mitra, A. et al. Development of a transgenic hairy root system in jute (Corchorus capsularis L.) with gusA reporter gene through Agrobacterium rhizogenes mediated co-transformation. Plant Cell Rep 30, 485–493 (2011). https://doi.org/10.1007/s00299-010-0957-y
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DOI: https://doi.org/10.1007/s00299-010-0957-y