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A MYB gene from wheat (Triticum aestivum L.) is up-regulated during salt and drought stresses and differentially regulated between salt-tolerant and sensitive genotypes

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Abstract

Crop adaptation to abiotic stresses requires alterations in expression of a large number of stress protection genes and their regulators, including transcription factors. In this study, the expression levels of ten MYB transcription factor genes from wheat (Triticum aestivum) were examined in two recombinant inbred lines contrasting in their salt tolerance in response to salt or drought stress. Quantitative RT-PCR analysis revealed that four MYB genes were consistently up-regulated in the seedling roots of both genotypes under short-term salt treatment. Three MYB genes were found to be up-regulated in both genotypes under long-term salt stress. One MYB gene was up-regulated in both genotypes under both short- and long-term salt stress. Of these salt up-regulated MYB genes, one MYB gene (TaMYBsdu1) was markedly up-regulated in the leaf and root of wheat under long-term drought stress. In addition, TaMYBsdu1 showed higher expression levels in the salt-tolerant genotype than in the susceptible genotype under salt stress. These data suggest that TaMYBsdu1 is a potentially important regulator involved in wheat adaptation to both salt and drought stresses.

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References

  • Abe H, Urao T, Ito T, Seki M, Shinozaki K, Yamaguchi-Shinozaki K (2003) Arabidopsis AtMYC2 (bHLH) and AtMYB2 (MYB) function as transcriptional activators in abscisic acid signaling. Plant Cell 15:63–78

    Article  CAS  PubMed  Google Scholar 

  • Bartels D, Sunkar R (2005) Drought and salt tolerance in plants. Crit Rev Plant Sci 24:23–58

    Article  CAS  Google Scholar 

  • Chen R, Ni Z, Nie X, Qin Y, Dong G, Sun Q (2005) Isolation and characterization of genes encoding Myb transcription factor in wheat (Triticum aestivum L.). Plant Sci 169:1146–1154

    Article  CAS  Google Scholar 

  • De Leonardis AM, Marone D, Mazzucotelli E, Neffar F, Rizza F, Di Fonzo N, Cattivelli L, Mastrangelo AM (2007) Durum wheat genes up-regulated in the early phases of cold stress are modulated by drought in a developmental and genotype dependent manner. Plant Sci 172:1005–1016

    Article  Google Scholar 

  • Kam J, Gresshoff P, Shorter R, Xue GP (2007) Expression analysis of RING zinc finger genes from Triticum aestivum and identification of TaRZF70 that contains four RING-H2 domains and differentially responds to water deficit between leaf and root. Plant Sci 173:650–659

    Article  CAS  Google Scholar 

  • Kam J, Gresshoff PM, Shorter R, Xue GP (2008) The Q-type C2H2 zinc finger subfamily of transcription factors in Triticum aestivum is predominantly expressed in roots and enriched with members containing an EAR repressor motif and responsive to drought stress. Plant Mol Biol 67:305–322

    Article  CAS  PubMed  Google Scholar 

  • Kawaura K, Mochida K, Ogihara Y (2008) Genome-wide analysis for identification of salt-responsive genes in common wheat. Funct Integr Genomics 8:277–286

    Article  CAS  PubMed  Google Scholar 

  • Liu Q, Kasuga M, Sakuma Y, Abe H, Miura S, Yamaguchi-Shinozaki K, Shinozaki K (1998) Two transcription factors, DREB1 and DREB2, with an EREBP/AP2 DNA binding domain separate two cellular signal transduction pathways in drought- and low-temperature-responsive gene expression, respectively, in Arabidopsis. Plant Cell 10:1391–1406

    Article  CAS  PubMed  Google Scholar 

  • Mare C, Mazzucotelli E, Crosatti C, Francia E, Stanca AM, Cattivelli L (2004) Hv-WRKY38: a new transcription factor involved in cold and drought-response in barley. Plant Mol Biol 55:399–416

    Article  CAS  PubMed  Google Scholar 

  • Mott IW, Wang RRC (2007) Comparative transcriptome analysis of salt-tolerant wheat germplasm lines using wheat genome arrays. Plant Sci 173:327–339

    Article  CAS  Google Scholar 

  • Novillo F, Alonso JM, Ecker JR, Salinas J (2004) CBF2/DREB1C is a negative regulator of CBF1/DREB1B and CBF3/DREB1A expression and plays a central role in stress tolerance in Arabidopsis. Proc Natl Acad Sci USA 101:3985–3990

    Article  CAS  PubMed  Google Scholar 

  • Olivares-Villegas JJ, Reynolds MP, McDonald GK (2007) Drought-adaptive attributes in the Seri/Babax hexaploid wheat population. Funct Plant Biol 34:189–203

    Article  Google Scholar 

  • Pfaffl MW (2001) A mathematical model for relative quantification of real-time RT-PCR. Nucleic Acid Res 29:2002–2007

    Google Scholar 

  • Riechmann JL, Heard J, Martin G, Reuber L, Jiang CZ, Keddie J, Adam L, Pineda O, Ratcliffe OJ, Samaha RR et al (2000) Arabidopsis transcription factors: genome-wide comparative analysis among eukaryotes. Science 290:2105–2110

    Article  CAS  PubMed  Google Scholar 

  • Sakuma Y, Maruyama K, Osakabe Y, Qin F, Seki M, Shinozaki K, Yamaguchi-Shinozaki K (2006) Functional analysis of an Arabidopsis transcription factor, DREB2A, involved in drought-responsive gene expression. Plant Cell 18:1292–1309

    Article  CAS  PubMed  Google Scholar 

  • Shaw LM, McIntyre CL, Gresshoff PM, Xue GP (2009) Members of the Dof transcription factor family in Triticum aestivum are associated with light-mediated gene regulation. Funct Integr Genomics 9:485–498

    Article  CAS  PubMed  Google Scholar 

  • Shinozaki K, Yamaguchi-Shinozaki K, Seki M (2003) Regulatory network of gene expression in the drought and cold stress responses. Curr Opin Plant Biol 6:410–417

    Article  CAS  PubMed  Google Scholar 

  • Singh K, Foley RC, Onate-Sanchez L (2002) Transcription factors in plant defense and stress responses. Curr Opin Plant Biol 5:430–436

    Article  CAS  PubMed  Google Scholar 

  • Stephenson TJ, McIntyre CL, Collet C, Xue GP (2007) Genome-wide identification and expression analysis of the NF-Y family of transcription factors in Triticum aestivum. Plant Mol Biol 65:77–92

    Article  CAS  PubMed  Google Scholar 

  • Tran LS, Nakashima K, Sakuma Y, Simpson SD, Fujita Y, Maruyama K, Fujita M, Seki M, Shinozaki K, Yamaguchi-Shinozaki K (2004) Isolation and functional analysis of Arabidopsis stress-inducible NAC transcription factors that bind to a drought-responsive cis-element in the early responsive to dehydration stress 1 promoter. Plant Cell 16:2481–2498

    Article  CAS  PubMed  Google Scholar 

  • Uno Y, Furihata T, Abe H, Yoshida R, Shinozaki K, Yamaguchi-Shinazachi K (2000) Arabidopsis basic leucine zipper transcription factors involved in an abscisic acid-dependent signal transduction pathways under drought and high-salinity conditions. Proc Natl Acad Sci USA 97:11632–11637

    Article  CAS  PubMed  Google Scholar 

  • Winter D, Vinegar B, Nahal H, Ammar R, Wilson GV, Provart NJ (2007) An “electronic fluorescent pictograph” browser for exploring and analyzing large-scale biological data sets. PLoS One 2:e718

    Article  PubMed  Google Scholar 

  • Xue GP (2003) The DNA-binding activity of an AP2 transcriptional activator HvCBF2 involved in regulation of low-temperature responsive genes in barley is modulated by temperature. Plant J 33:373–383

    Article  CAS  PubMed  Google Scholar 

  • Xue GP, Loveridge CW (2004) HvDRF1 is involved in abscisic acid mediated gene regulation in barley and produces two forms of AP2 transcriptional activators, interacting preferably with a CT rich element. Plant J 37:326–339

    Article  CAS  PubMed  Google Scholar 

  • Xue GP, Bower NI, McIntyre CL, Riding GA, Kazan K, Shorter R (2006) TaNAC69 from the NAC superfamily of transcription factors is up-regulated by abiotic stresses in wheat and recognizes two consensus DNA-binding sequences. Funct Plant Biol 33:43–57

    Article  CAS  Google Scholar 

  • Xue GP, McIntyre CL, Jenkins CLD, Glassop D, van Herwaarden AF, Shorter R (2008) Molecular dissection of variation in carbohydrate metabolism related to water soluble carbohydrate accumulation in stems of wheat (Triticum aestivam L.). Plant Physiol 146:441–454

    Article  CAS  PubMed  Google Scholar 

  • Zeller G, Henz SR, Widmer CK, Sachsenberg T, Ratsch G, Weigel D, Laubinger S (2009) Stress-induced changes in the Arabidopsis thaliana transcriptome analyzed using whole-genome tiling arrays. Plant J 58:1068–1082

    Article  CAS  PubMed  Google Scholar 

  • Zhong R, Lee C, Zhou J, McCarthy RL, Ye Z-H (2008) A battery of transcription factors involved in the regulation of secondary cell wall biosynthesis in Arabidopsis. Plant Cell 20:2763–2782

    Article  CAS  PubMed  Google Scholar 

  • Zhu JK (2002) Salt and drought stress signal transduction in plants. Annu Rev Plant Biol 53:247–273

    Article  CAS  PubMed  Google Scholar 

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Acknowledgments

We wish to thank Krassen Dimitrov for financial support for part of this study, and Nasser Sewelam, Shazia Iram and other members of the Schenk laboratory for technical assistance. We would like to thank The University of Tehran for providing a doctoral fellowship to MR.

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Correspondence to Peer M. Schenk.

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Communicated by P. Lakshmanan.

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Rahaie, M., Xue, GP., Naghavi, M.R. et al. A MYB gene from wheat (Triticum aestivum L.) is up-regulated during salt and drought stresses and differentially regulated between salt-tolerant and sensitive genotypes. Plant Cell Rep 29, 835–844 (2010). https://doi.org/10.1007/s00299-010-0868-y

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  • DOI: https://doi.org/10.1007/s00299-010-0868-y

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