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Expression analysis of a plum pathogenesis related 10 (PR10) protein during brown rot infection

  • Physiology and Biochemistry
  • Published:
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Abstract

Plant PR10 is one of the pathogenesis related proteins, induced upon exposure to different stress conditions including fungal infection. PR10 proteins have been implicated in fungal disease resistance in some species; however its transcriptional regulation is not well understood. In the present work we cloned a PR10 gene from European plums (Prunus domestica L.) and monitored the quantitative changes in its transcript levels as a result of fungal infection in two varieties. We also studied the possible involvement of the membrane degrading enzyme phospholipase D-alpha (PLDα). In the susceptible variety, ‘Veeblue’, infection with the brown rot fungus Monilinia fructicola induced PLDα and PR10 expression, while in the resistant variety, ‘Violette’, a constitutive expression of PLDα and PR10 transcripts levels were observed. Resistance to M. fructicola also coincides with a sharp decrease in the expression of ABI1, a protein phosphatase and elevated hydrogen peroxide content after infection. Further, inhibition of PLDα by hexanal treatment, up-regulated ABI1 and decreased PR10 expression, suggesting a possible relationship between the two. We further confirm these results in Arabidopsis abi1 mutant that shows a higher level of PR10 transcripts.

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Acknowledgements

We thank Dr. Peter Pauls, University of Guelph, for providing generous use of real-time-PCR equipment Lana Wainman, AAFC, Vineland Station, Peter Alm and Jennifer Street for technical assistance. We also to thank the Early Researchers Award of Ontario Ministry of Innovation, Canadian Foundation for Innovation, Ontario Innovation Trust, Ontario Ministry of Agriculture, Food and Rural Affairs and Niagara Tender Fruit Marketing Board for the financial assistance to S. Jayasankar.

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Authors and Affiliations

  1. Department of Plant Agriculture, University of Guelph, 4890 Victoria Avenue North, P.O. Box 7000, Vineland Station, ON, L0R2E0, Canada

    Ashraf El-kereamy & S. Jayasankar

  2. Department of Plant Agriculture, University of Guelph, Guelph, ON, N1G2W1, Canada

    Ali Taheri & Gopinadhan Paliyath

  3. Agriculture and Agri-Food Canada, Southern Crop Protection and Food Research Centre, 4902 Victoria Avenue North, P.O. Box 6000, Vineland Station, ON, L0R2E0, Canada

    Deena Errampalli

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  1. Ashraf El-kereamy
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  2. S. Jayasankar
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  3. Ali Taheri
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  4. Deena Errampalli
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  5. Gopinadhan Paliyath
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Correspondence to S. Jayasankar.

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Communicated by K. Kamo.

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El-kereamy, A., Jayasankar, S., Taheri, A. et al. Expression analysis of a plum pathogenesis related 10 (PR10) protein during brown rot infection. Plant Cell Rep 28, 95–102 (2009). https://doi.org/10.1007/s00299-008-0612-z

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  • DOI: https://doi.org/10.1007/s00299-008-0612-z

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