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Production and characterization of intertribal somatic hybrids of Raphanus sativus and Brassica rapa with dye and medicinal plant Isatis indigotica

  • Genetic Transformation and Hybridization
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Abstract

Intertribal somatic hybrids of Raphanus sativus (2n = 18, RR) and Brassica rapa spp. chinensis (2n = 20, AA) with the dye and medicinal plant Isatis indigotica (2n = 14, I I) were firstly obtained by polyethylene glycol-induced symmetric fusions of mesophyll protoplasts. One mature hybrid with R. sativus established in field had intermediate morphology but was totally sterile. It had the expected chromosome number (2n = 32, RRI I) and parental chromosomes were distinguished by genomic in situ hybridization (GISH) analysis, and these chromosomes were paired as 16 bivalents in pollen mother cells (PMCs) at diakinesis and mainly segregated equally as 16:16 at anaphase I (A I), but the meiotic disturbance in second division was obvious. Five mature hybrids with B. rapa established in field were morphologically intermediate but showed some differences in phenotypic traits and fertility, two were partially fertile. Cytological and GISH investigations revealed that these hybrids had 2n = 48 with AAIIII complement and their PMCs showed normal pairing of 24 bivalents and mainly equal segregation 24:24, but meiotic abnormalities of lagging chromosomes and micronuclei appeared frequently during second divisions. AFLP analysis showed that all of these hybrids had mainly the DNA banding pattern from the addition of two parents plus some alterations. Some hybrids should be used for the genetic improvement of crops and the dye and medicinal plant.

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Acknowledgements

The study was supported by grants from Natural Science Foundation of China (30571033) and by PCSIRT (IRT0442), nyhyzx07-054.

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Authors and Affiliations

  1. National Key Laboratory of Crop Genetic Improvement, National Center of Crop Molecular Breeding Technology, National Center of Oil Crop Improvement (Wuhan), College of Plant Science and Technology, Huazhong Agricultural University, 430070, Wuhan, People’s Republic of China

    Yuqin Tu, Jian Sun, Yan Liu, Xianhong Ge, Zhigang Zhao, Xingcheng Yao & Zaiyun Li

  2. Oil Crops Research Institute, Chinese Academy of Agricultural Sciences, 430062, Wuhan, People’s Republic of China

    Jian Sun

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  1. Yuqin Tu
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  2. Jian Sun
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  3. Yan Liu
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Correspondence to Zaiyun Li.

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Communicated by K. Toriyama.

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Tu, Y., Sun, J., Liu, Y. et al. Production and characterization of intertribal somatic hybrids of Raphanus sativus and Brassica rapa with dye and medicinal plant Isatis indigotica . Plant Cell Rep 27, 873–883 (2008). https://doi.org/10.1007/s00299-008-0513-1

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