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Stress-induced expression of choline oxidase in potato plant chloroplasts confers enhanced tolerance to oxidative, salt, and drought stresses

  • Genetic Transformation and Hybridization
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Abstract

Transgenic potato plants (Solanum tuberosum L. cv. Superior) with the ability to synthesize glycinebetaine (GB) in chloroplasts (referred to as SC plants) were developed via the introduction of the bacterial choline oxidase (codA) gene under the control of an oxidative stress-inducible SWPA2 promoter. SC1 and SC2 plants were selected via the evaluation of methyl viologen (MV)-mediated oxidative stress tolerance, using leaf discs for further characterization. The GB contents in the leaves of SC1 and SC2 plants following MV treatment were found to be 0.9 and 1.43 μmol/g fresh weight by HPLC analysis, respectively. In addition to reduced membrane damage after oxidative stress, the SC plants evidenced enhanced tolerance to NaCl and drought stress on the whole plant level. When the SC plants were subjected to two weeks of 150 mM NaCl stress, the photosynthetic activity of the SC1 and SC2 plants was attenuated by 38 and 27%, respectively, whereas that of non-transgenic (NT) plants was decreased by 58%. Under drought stress conditions, the SC plants maintained higher water contents and accumulated higher levels of vegetative biomass than was observed in the NT plants. These results indicate that stress-induced GB production in the chloroplasts of GB non-accumulating plants may prove useful in the development of industrial transgenic plants with increased tolerance to a variety of environmental stresses for sustainable agriculture applications.

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Acknowledgments

This work was supported by grants from the Biogreen 21 program (Code # 20070301034015), Rural Development Administration, Korea, from the Environmental Biotechnology National Core Research Center, KOSEF/MOST, and from the Korea Foundation for International Cooperation of Science and Technology (KIOCS).

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Authors and Affiliations

  1. Department of Biological Sciences, Korea Advanced Institute of Science and Technology (KAIST), Daejon, 305-701, South Korea

    Raza Ahmad & Won-Il Chung

  2. Environmental Biotechnology Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Eoeun-dong 52, Yuseong, Daejon, 305-806, South Korea

    Raza Ahmad, Myoung Duck Kim, Kyung-Hwa Back, Hee-Sik Kim, Haeng-Soon Lee & Sang-Soo Kwak

  3. Plant Genomics Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Eoeun-dong 52, Yuseong, Daejon, 305-806, South Korea

    Suk-Yoon Kwon

  4. National Institute for Basic Biology, Myodaiji, Okazaki, 444-8585, Japan

    Norio Murata

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  1. Raza Ahmad
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  2. Myoung Duck Kim
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  3. Kyung-Hwa Back
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  7. Norio Murata
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  8. Won-Il Chung
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  9. Sang-Soo Kwak
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Correspondence to Won-Il Chung or Sang-Soo Kwak.

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Communicated by J.R. Liu.

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Ahmad, R., Kim, M.D., Back, KH. et al. Stress-induced expression of choline oxidase in potato plant chloroplasts confers enhanced tolerance to oxidative, salt, and drought stresses. Plant Cell Rep 27, 687–698 (2008). https://doi.org/10.1007/s00299-007-0479-4

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  • DOI: https://doi.org/10.1007/s00299-007-0479-4

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