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Transformation of a muskmelon ‘Galia’ hybrid parental line (Cucumis melo L. var. reticulatus Ser.) with an antisense ACC oxidase gene

  • Genetic Transformation and Hybridization
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Abstract

‘Galia’ muskmelon (Cucumis melo L. var. reticulatus Ser.) has been recalcitrant to transformation by Agrobacterium tumefaciens. Transformation of the ‘Galia’ male parental line, ‘Krymka’, with an ACC oxidase (CMACO-1) gene in antisense orientation is described herein. Explants were transformed using A. tumefaciens strain ABI, which contained a vector pCmACO1-AS plasmid, bearing an antisense gene of CMACO-1 and the CP4 syn gene (glyphosate-tolerance). Both CMACO-1 and CP4 syn genes were assessed by a polymerase chain reaction method. Flow cytometry analysis was performed to determine plant ploidy level of primary transformants. Two completely diploid independent transgenic plants were obtained. Southern blot and segregation analysis in the T1 generation determined that each independent transgenic line had one single insertion of the transgene. These transgenic muskmelon male parental lines have potential for use in the production of ‘Galia’ F1 hybrids with improved shelf life.

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Abbreviations

ACC:

1-Aminocyclopropane-1-carboxylic acid

ACO-1:

ACC oxidase-1

CMACO-1:

Cucumis melo ACO-1

BA:

6-Benzylaminopurine

IAA:

Indole-3-acetic acid

MS:

Murashige and Skoog medium

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Acknowledgements

This work was supported by a USDA/CBAG PL-89-106 grant. We gratefully acknowledge Dawn Bies for the technical help for the PCR assays and Brian Kevany for the Southern blot analysis

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Author notes

  1. Joseph Ciardi

    Present address: BioRexis Pharmaceutical Corporation, 3400 Horizon Drive, King of Prussia, PA, 19406, USA

Authors and Affiliations

  1. Horticultural Sciences Department, University of Florida, IFAS, Gainesville, FL, 32611, USA

    Hector G. Nuñez-Palenius, Daniel J. Cantliffe, Don J. Huber, Joseph Ciardi & Harry J. Klee

  2. Genetic Engineering Department, CINVESTAV, Irapuato, Gto., CP, 36500, Mexico

    Hector G. Nuñez-Palenius

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  1. Hector G. Nuñez-Palenius
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  2. Daniel J. Cantliffe
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  3. Don J. Huber
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Correspondence to Daniel J. Cantliffe.

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Communicated by P. Ozias-Akins

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Nuñez-Palenius, H.G., Cantliffe, D.J., Huber, D.J. et al. Transformation of a muskmelon ‘Galia’ hybrid parental line (Cucumis melo L. var. reticulatus Ser.) with an antisense ACC oxidase gene. Plant Cell Rep 25, 198–205 (2006). https://doi.org/10.1007/s00299-005-0042-0

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  • DOI: https://doi.org/10.1007/s00299-005-0042-0

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