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Transient expression of Arabidopsis thaliana ascorbate peroxidase 3 in Nicotiana benthamiana plants infected with recombinant potato virus X

  • Biotic and Abiotic Stress
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Abstract

We have explored the transient over-expression of Arabidopsis thaliana ascorbate peroxidase 3 (APX3) in Nicotiana benthamiana using a viral vector based on the potato virus X (PVX). Plants infected with a PVX:APX3 hybrid had a similar progression of viral particles compared to control plants infected with a PVX:GFP hybrid, indicating that infection was not affected by the over-expression of heterologous APX3. Our results also showed that in PVX:APX3-infected plants, the hybrid virus directed a high level of APX3 expression and the recombinant protein was functional, as inferred from the higher APX activity compared to mock and PVX:GFP hybrid-infected plants. The PVX recombinant expression system used is a simple and quick method for transient expression of heterologous APXs, which are expected to suffer specific processing in plant cells.

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Acknowledgements

We are very grateful to Dr Trelease and Dr Dalton for providing the primary immunoserum anti- glyoxysomal/peroxisomal cucumber APX and anti-cytosolic soybean APXs, respectively. We also thank Dr D. Baulcome for providing the pC2C2S vector and for technical advice.

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Authors and Affiliations

  1. Departamento de Biología Vegetal, Facultad de Ciencias Ambientales, Universidad de Castilla la Mancha, Campus de la Fábrica de Armas, 45003, Toledo, Spain

    C. Escobar

  2. John Innes Centre, Norwich Research Park, Colney Lane, Norwich, NR4 7UH, UK

    C. Escobar, L. E. Hernández, A. Jiménez, G. Creissen & P. M. Mullineaux

  3. Sainsbury Laboratory, John Innes Centre, Colney Lane, Norwich, NR4 7UH, UK

    M. T. Ruiz

Authors
  1. C. Escobar
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  2. L. E. Hernández
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  3. A. Jiménez
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  4. G. Creissen
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  5. M. T. Ruiz
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  6. P. M. Mullineaux
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Corresponding author

Correspondence to C. Escobar.

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Communicated by L Peña

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Escobar, C., Hernández, L.E., Jiménez, A. et al. Transient expression of Arabidopsis thaliana ascorbate peroxidase 3 in Nicotiana benthamiana plants infected with recombinant potato virus X. Plant Cell Rep 21, 699–704 (2003). https://doi.org/10.1007/s00299-002-0570-9

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  • DOI: https://doi.org/10.1007/s00299-002-0570-9

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