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Study of serial serum myeloid-related protein 8/14 as a sensitive biomarker in Takayasu arteritis: a single centre study

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Abstract

The aim of the study was to explore utility of serial serum myeloid-related protein 8/14 (MRP8/14) as a biomarker of clinical disease activity and angiographic progression in Takayasu arteritis (TA). Serum MRP8/14 levels were assayed by commercial ELISA for 85 TA patients and 24 healthy controls at baseline, and for 56 and 21 TA patients during follow-up visits R1 and R2, respectively. Disease was categorised as active, indeterminate and stable according to Indian Takayasu Arteritis score (ITAS 2010), ITAS-A(CRP) and angiography. Patients were divided into responders and non-responders/relapsers based on treatment response. Non-parametric tests were used for inter-group comparisons at baseline and during follow-up time points. Generalised Estimating Equation was used to study association between changes in serial MRP8/14 levels and disease activity. At baseline, median MRP8/14 levels were higher in patients with TA than healthy controls [7353 (4524 to11283) vs 4896 (3194 to 8474.5) ng/ml, p = 0.011]. Patients with active disease had higher levels [8552 (5463–12488)] than stable disease [5292.5 (3140.5–7310)], p = 0.002, and healthy controls [4896 (3194–8474.5)], p = 0.001. Changes in serial MRP8/14 level were associated with changes in disease activity, independent of steroid dose, p = 0.000. At R1, MRP 8/14 levels were lower than baseline in responders (n = 38) [9146.0 (6296.8–13693.8) vs 6501 (4314.8–8304.5), p = 0.004], but did not change in non-responders/relapsers (n = 14) [6693.5(4210.8–10516.3) vs 7755.0(5342–10741.0), p = 0.42]. Similar trend was observed at R2. MRP8/14 levels increased during follow-up in 66% and 26.3% of angiographic progressors and non-progressors, respectively. MRP8/14 in TA may act as a novel biomarker with prognostic implications.

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Authors and Affiliations

  1. Department of Clinical Immunology and Rheumatology, Christian Medical College, Vellore, 632004, India

    Ruchika Goel, Aswin Nair, Jayakanthan Kabeerdoss, Hindhumathi Mohan & Debashish Danda

  2. Department of Biostatistics, Christian Medical College, Vellore, 632004, India

    Visalakshi Jeyaseelan

  3. Department of Cardiology, Christian Medical College, Vellore, 632004, India

    George Joseph

Authors
  1. Ruchika Goel
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  2. Aswin Nair
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  3. Jayakanthan Kabeerdoss
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  4. Hindhumathi Mohan
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  5. Visalakshi Jeyaseelan
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  6. George Joseph
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  7. Debashish Danda
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Contributions

RG has contributed to designing and execution of the study. RG, AN, JK, GJ and DD have contributed to preparation and critical review of manuscript. HM has assisted in MRP 8/14 ELISA and VJ has helped in statistical analysis.

Corresponding author

Correspondence to Debashish Danda.

Ethics declarations

The authors have no potential conflicts of interest. All procedures performed in this study were in accordance with the ethical standards of institutional research committee and complied with the 1994 Helsinki Declaration and its amendments or comparable ethical standards. The patients were recruited in this study after written informed consent.

Funding

This study was supported by a research grant to Dr. Aswin Nair from the Indian Rheumatology Association.

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Goel, R., Nair, A., Kabeerdoss, J. et al. Study of serial serum myeloid-related protein 8/14 as a sensitive biomarker in Takayasu arteritis: a single centre study. Rheumatol Int 38, 623–630 (2018). https://doi.org/10.1007/s00296-017-3881-4

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  • DOI: https://doi.org/10.1007/s00296-017-3881-4

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