Abstract
Rheumatoid arthritis is an autoimmune disease that affects several organs and tissues, predominantly the synovial joints. The pathogenesis of this disease is not completely understood, which maybe involved in the genomic variations, gene expression, protein translation and post-translational modifications. These system variations in genomics, transcriptomics and proteomics are dynamic in nature and their crosstalk is overwhelmingly complex, thus analyzing them separately may not be very informative. However, various ‘-omics’ techniques developed in recent years have opened up new possibilities for clarifying disease pathways and thereby facilitating early diagnosis and specific therapies. This review examines how recent advances in the fields of genomics, transcriptomics and proteomics have contributed to our understanding of rheumatoid arthritis.
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Abbreviations
- RA:
-
Rheumatoid arthritis
- FLS:
-
Fibroblast-like synovial cells
- HC:
-
Healthy control
- IFN:
-
Interferon
- OA:
-
Osteoarthritis
- PBMC:
-
Peripheral blood mononuclear cell
- RASF:
-
Rheumatoid arthritis synovial fibroblast
- RNAseq:
-
RNA sequencing
- SF:
-
Synovial fibroblast
- SPP1:
-
Secreted phosphoprotein 1
- 2DE:
-
Two-dimensional gel electrophoresis
- 2D-LC:
-
Two-dimensional liquid chromatography
- DIGE:
-
Difference gel electrophoresis
- iTRAQ:
-
Isobaric tagging for relative and absolute quantitation
- LC–MS/MS:
-
Liquid chromatography coupled to tandem mass spectrometry
- MALDI-TOF MS:
-
Matrix-assisted laser desorption ionization-time of-flight mass spectrometry
- MSC:
-
Mesenchymal stem cell
- SLE:
-
Systemic lupus erythematosus
- SpA:
-
Spondyloarthropathy
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Acknowledgements
This research was supported by the China Scholarship Council and the International Training Program of High-Level Talents in Henan Province.
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All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards.
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Song, X., Lin, Q. Genomics, transcriptomics and proteomics to elucidate the pathogenesis of rheumatoid arthritis. Rheumatol Int 37, 1257–1265 (2017). https://doi.org/10.1007/s00296-017-3732-3
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DOI: https://doi.org/10.1007/s00296-017-3732-3