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Morphological adaptation of muscle collagen and receptor of advanced glycation end product (RAGE) in osteoarthritis patients with 12 weeks of resistance training: influence of anti-inflammatory or glucosamine treatment

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Abstract

The aim of this study was to investigate the effect of 12-week resistance training on morphological presence of collagen and RAGE (receptor for advanced glycation end products) in skeletal muscle of patients with knee osteoarthritis (OA). Little is known about the influence of exercise on the skeletal muscle matrix that supports joints affected by OA mainly when it is associated with medication taken by OA patients (non-steroid anti-inflammatory drugs (NSAID) and glucosamine). A biopsy was collected from the vastus lateralis muscle in all patients before and after 12-week period of training. The patients (age 55–69 years) were divided into three groups, treated with NSAID, glucosamine or placebo. In addition, the muscle samples were analysed by immunohistochemistry for collagen types, RAGE and capillaries ratio. An increment in immunoreactivity for type IV collagen after the training period was observed in 72 % of all biopsies when compared with their respective baseline samples. Reduced immunoreactivity of collagen type I was observed in all patients treated with glucosamine. A significant increase with training in the amount of RAGE was detected in the placebo group only (p < 0.05). Comparison of post-treatment states indicated significant differences between the placebo and glucosamine group data, demonstrating increased levels in the placebo group (p < 0.05). These findings suggest a basement membrane remodelling in favour of a strengthened extracellular matrix surrounding individual muscle fibres after 12 weeks of resistance training. Glucosamine with training appeared to attenuate RAGE accumulation more than was seen with NSAID or placebo in skeletal muscle of OA patients.

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Acknowledgments

A.C. Mattiello-Sverzut was recipient of postdoctoral fellowships from Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES), Brazil (Grants n.0641.08.1). This work was supported by the Danish Rheumatism Association, Nordea Foundation (Healthy Aging grant), Danish Ministry of Health, IMK (Ib Mogens Kristiansen) Almene Fond and MYOAGE (nr. 223576) funded by the European Commission under FP7.

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The authors report no conflicts of interest. The authors alone are responsible for the content and writing of the paper.

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Authors and Affiliations

  1. Department of Biomechanics, Medicine and Rehabilitation of the Locomotor Apparatus, Ribeirão Preto School of Medicine, University of São Paulo, Av. Bandeirantes, 3900, Ribeirão Preto, SP, 14049-900, Brazil

    Ana Claudia Mattiello-Sverzut

  2. Department of Orthopaedic Surgery M, Institute of Sports Medicine, Bispebjerg Hospital, and Center for Healthy Aging, Faculty of Health Sciences, University of Copenhagen, Building 8, 1st Floor, Bispebjerg Bakke 23, Copenhagen, NV, 2400, Denmark

    Susanne G. Petersen, Michael Kjaer & Abigail L. Mackey

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  1. Ana Claudia Mattiello-Sverzut
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  2. Susanne G. Petersen
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  4. Abigail L. Mackey
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Correspondence to Ana Claudia Mattiello-Sverzut.

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Mattiello-Sverzut, A.C., Petersen, S.G., Kjaer, M. et al. Morphological adaptation of muscle collagen and receptor of advanced glycation end product (RAGE) in osteoarthritis patients with 12 weeks of resistance training: influence of anti-inflammatory or glucosamine treatment. Rheumatol Int 33, 2215–2224 (2013). https://doi.org/10.1007/s00296-013-2698-z

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