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Lack of association of TLR4 gene Asp299Gly and Thr399Ile polymorphisms with rheumatoid arthritis in Chinese Han population of Yunnan Province

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Abstract

Rheumatoid arthritis (RA) is a systemic autoimmune disease characterized by chronic inflammation of synovium and subsequent joint destruction. Recently, genetic polymorphisms within the toll-like receptor 4 (TLR4) genes have been reported to be associated with RA. To analyze the association between the genetic polymorphisms within TLR4 gene and the susceptibility to RA in Chinese people, two functional variants, Asp299Gly and Thr399Ile, in the TLR4 gene were genotyped using polymerase chain reaction–restriction fragment length polymorphism (PCR–RFLP) and direct sequencing techniques from 213 RA patients and 247 ethnically matched controls. None polymorphisms of Asp299Gly and Thr399Ile were detected in all RA cases and controls, which indicates that there is no relevance between these two SNPs and RA in the Chinese Han population. Further studies with extended single nucleotide polymorphisms (SNP) should be performed.

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Acknowledgments

This study was supported by The National Nature Science Foundation of China (30660175), Yunnan Province Young Scientist Project (2009CI044), National Undergraduate Students Research Training Found from Ministry of Education of China(81067301), National Found for Fostering Talents of Basic Science (J0730652) and Research Training Found from Yunnan University Experiment Teaching Center of Life Sciences.

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Authors and Affiliations

  1. Key Laboratory of Bioresources Conservation and Utilization and Human Genetics Center of Yunnan University, 650091, Kunming, Yunnan, People’s Republic of China

    Bingrong Zheng, Jiao Qin, Ruoyu Zhou, Jingru Shao & Chunjie Xiao

  2. Kunhua Affiliated Hospital of Kunming Medical College, 650032, Kunming, Yunnan, People’s Republic of China

    Qin Li, Tao Shou & Yan Yang

  3. Yan’an Affiliated Hospital of Kunming Medical College, 650051, Kunming, Yunnan, People’s Republic of China

    Chuanyu Wei

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  1. Bingrong Zheng
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  2. Qin Li
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  3. Chuanyu Wei
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  4. Jiao Qin
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  5. Tao Shou
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  9. Chunjie Xiao
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Correspondence to Bingrong Zheng or Chunjie Xiao.

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Bingrong Zheng and Qin Li are contributed equally to this work.

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Zheng, B., Li, Q., Wei, C. et al. Lack of association of TLR4 gene Asp299Gly and Thr399Ile polymorphisms with rheumatoid arthritis in Chinese Han population of Yunnan Province. Rheumatol Int 30, 1249–1252 (2010). https://doi.org/10.1007/s00296-010-1400-y

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  • DOI: https://doi.org/10.1007/s00296-010-1400-y

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