Abstract
Measuring soft tissue thickness is an important step in rheumatoid disease research. The severity of mouse footpad swelling can be used as an indicator of disease progression. A noncontact footpad thickness assay, simplified geometry measurement system (SGMS), was developed that was able to reduce both intra- and interobserver variances during measurements. Three materials with five objects each were used in this study: hard blocks, soft sponges and mouse footpads. Thicknesses were measured using calipers or the SGMS. In the measurement of the hard block, there was no difference in measurement errors between calipers and SGMS. For the mouse footpad thickness, there was significant difference in intraobserver variances among three observers and a significant difference of interobserver variances between calipers and SGMS. In conclusions, this noncontact assay is reliable and highly reproducible for the assessment of inflammatory reactions when results are expressed as a gradual increase in footpad thickness.
References
Feldmann M, Brennan FM, Maini RN (1996) Rheumatoid arthritis. Cell 85:307–310. doi:10.1016/S0092-8674(00)81109-5
Nandakumar KS, Bäcklund J, Vestberg M et al (2004) Collagen type II (CII)-specific antibodies induce arthritis in the absence of T or B cells but the arthritis progression is enhanced by CII-reactive T cells. Arthritis Res Ther 6:R544–R550. doi:10.1186/ar1217
Choe J-Y, Crain B, Wu SR et al (2003) Interleukin 1 receptor dependence of serum transferred arthritis can be circumvented by Toll-like receptor 4 signaling. J Exp Med 197:537–542. doi:10.1084/jem.20021850
Kornaat PR, Koo S, Andriacchi TP, Bloem JL, Gold GE (2006) Comparison of quantitative cartilage measurements acquired on two 30T MRI systems from different manufacturers. Magn Reson Imaging 23:770–773. doi:10.1002/jmri.20561
Terato K, Hasty KA, Reife RA et al (1992) Induction of arthritis with monoclonal antibodies to collagen. J Immunol 148:2103–2108
Terato K, Harper DS, Griffiths MM et al (1995) Collagen-induced arthritis in mice: synergistic effect of E. coli lipopolysaccharide bypasses epitope specificity in the induction of arthritis with monoclonal antibodies to type II collagen. Autoimmunity 22:137–147. doi:10.3109/08916939508995311
Healy MJR (1989) Measuring errors. Stat Med 8:893–906. doi:10.1002/sim.4780080802
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Chia, WT., Lin, CF.J., Yeh, LT. et al. A noncontact footpad thickness assay to evaluate rheumatoid disease. Rheumatol Int 30, 547–550 (2010). https://doi.org/10.1007/s00296-009-1261-4
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DOI: https://doi.org/10.1007/s00296-009-1261-4