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Rae1-mediated nuclear export of Rnc1 is an important determinant in controlling MAPK signaling

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Abstract

In eukaryotic cells, RNA binding proteins (RBPs) play critical roles in regulating almost every aspect of gene expression, often shuttling between the nucleus and the cytoplasm. They are also key determinants in cell fate via controlling the target mRNAs under the regulation of various signaling pathways in response to environmental stresses. Therefore, understanding the mechanisms that couple the location of mRNA and RBPs is a major challenge in the field of gene expression and signal responses. In fission yeast, a KH-type RBP Rnc1 negatively regulates MAPK signaling activation via mRNA stabilization of the dual-specificity MAPK phosphatase Pmp1, which dephosphorylates MAPK Pmk1. Rnc1 also serves as a target of MAPK phosphorylation, which makes a feedback loop mediated by an RBP. We recently discovered that the nuclear export of Rnc1 requires mRNA-binding ability and the mRNA export factor Rae1. This strongly suggested the presence of an mRNA-export system, which recognizes the mRNA/RBP complex and dictates the location and post-transcriptional regulation of mRNA cargo. Here, we briefly review the known mechanisms of general nuclear transporting systems, with an emphasis on our recent findings on the spatial regulation of Rnc1 and its impact on the regulation of the MAPK signal transduction cascade.

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Acknowledgements

We would like to thank William Figoni for critical reading of the manuscript. We also would like to express our gratitude to members of the Laboratory of Molecular Pharmacogenomics for their support. This study was supported by a Grant-in-Aid for Japan Society for the Promotion of Science (JSPS) Fellows (22∙5817) (to R. Satoh), and Scientific Research on Innovative Areas ‘RNA regulation’ (20112006) from the Ministry of Education, Culture, Sports, Science and Technology (MEXT) of Japan (to R. Sugiura). This study was also supported by the MEXT-Supported Program for the Strategic Research Foundation at Private Universities, 2014–2018 (S1411037).

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  1. Laboratory of Molecular Pharmacogenomics, Department of Pharmaceutical Sciences, Kindai University, Higashiosaka City, Osaka, 577-8502, Japan

    Ryosuke Satoh, Kanako Hagihara & Reiko Sugiura

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  1. Ryosuke Satoh
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  2. Kanako Hagihara
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  3. Reiko Sugiura
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Correspondence to Reiko Sugiura.

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Communicated by M. Kupiec.

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Satoh, R., Hagihara, K. & Sugiura, R. Rae1-mediated nuclear export of Rnc1 is an important determinant in controlling MAPK signaling. Curr Genet 64, 103–108 (2018). https://doi.org/10.1007/s00294-017-0732-5

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