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Analysis of manganese-regulated gene expression in the ligninolytic basidiomycete Ceriporiopsis subvermispora

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Abstract

In this work, we explore the use of the unbiased cDNA–AFLP strategy to identify genes involved in Mn2+ homeostasis in Ceriporiopsis subvermispora. In this ligninolytic white-rot fungus, whose genome has not yet been sequenced, three Mn peroxidase genes responding to Mn2+ have been characterized. Using cDNA–AFLP to identify transcript-derived fragments (TDFs), a total of 37 differentially expressed cDNA fragments were identified by comparing band intensities among cDNA–AFLP patterns obtained from mycelia from cultures supplemented with different concentrations of Mn2+. Of 21 differentially expressed TDFs, nine were classified as upregulated, five as downregulated and seven as unregulated. Of these, six upregulated and two downregulated TDFs were selected for further characterization. The expected TDFs for the known Mn peroxidases were not isolated, but several genes encoding proteins related to protein sorting, storage and excretion of excess Mn2+ were identified. Transcripts induced under Mn2+ supplementation exhibited homologies to the elongation factor eEF3, a HDEL sequence binding protein and the ARD1 subunit of the N-acetyltransferase complex, among others. Overall, the results obtained in this study suggest a complex picture of Mn2+ homeostasis and provide the possibility to search for common regulatory elements in the promoters of the novel putatively identified genes.

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Acknowledgments

We thank Paulo Canessa for critical reading and comments on the manuscript, and for his assistance during this study. We also thank Ares Tirado for his collaboration in the preparation of the figures. This work was financed and supported by FONDECYT Project No. 1070588 and the Millennium Institute for Applied and Fundamental Biology Project No. P04-007-F.

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Author notes

  1. Matías Gutiérrez

    Present address: Anita Sil Lab, Department of Microbiology and Immunology, University of California, San Francisco, 513 Parnassus Ave. Room S-472, San Francisco, CA, 94143-0414, USA

  2. Luis Alejandro Rojas

    Present address: Department of Biochemistry, School of Medicine, New York University, 522 First Avenue Room 207, New York, NY, 10016, USA

Authors and Affiliations

  1. Laboratorio de Bioquímica, Departamento de Bioquímica y Biología Molecular, Facultad de Ciencias Químicas y Farmacéuticas, Universidad de Chile, Vicuña Mackenna 20, Providencia, Santiago, Chile

    Matías Gutiérrez, Luis Alejandro Rojas, Daniela Seelenfreund & Sergio Lobos

  2. Millennium Institute for Fundamental and Applied Biology, Santiago, Chile

    Matías Gutiérrez, Luis Alejandro Rojas, Rodrigo Mancilla-Villalobos, Rafael Vicuña & Sergio Lobos

  3. Departamento de Genética Molecular y Microbiología, Facultad de Ciencias Biológicas, Pontificia Universidad Católica de Chile, Santiago, Chile

    Rodrigo Mancilla-Villalobos & Rafael Vicuña

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  1. Matías Gutiérrez
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  2. Luis Alejandro Rojas
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  6. Sergio Lobos
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Corresponding author

Correspondence to Sergio Lobos.

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Communicated by U. Kües.

M. Gutiérrez and L. A. Rojas contributed equally to this work.

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Gutiérrez, M., Rojas, L.A., Mancilla-Villalobos, R. et al. Analysis of manganese-regulated gene expression in the ligninolytic basidiomycete Ceriporiopsis subvermispora . Curr Genet 54, 163–173 (2008). https://doi.org/10.1007/s00294-008-0209-7

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