Abstract.
We investigated a DNA fragment and its flanking region deleted in the spontaneous Pi-a virulent mutant of Magnaporthe grisea Ina168. A new transposon-like sequence was identified from a region adjacent to the deleted fragment and was named Occan. Occan contained a 2,259-bp ORF interrupted by one 63-bp intron and had both a TA dinucleotide and 77 bp of perfect inverted repeats at both termini, without direct repeats. These features indicated that Occan is a member of the Fot1 family. RT-PCR analysis confirmed the expression of the putative transposase and the presence of an intron. Southern analysis of pulse-field gel electrophoresis-separated chromosomes indicated that Occan was dispersed in all chromosomes of the rice pathogen, Ina168. Copy numbers of Occan were also preserved in a host-specific manner amongst M. grisea isolates. In particular, rice pathogens contained a large number of the element inserted into their genome. Phylogenetic analysis with other known members of the Fot1 family revealed that Occan was dissimilar to any other known elements and it is thus proposed that Occan be separated to a new subfamily.
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Acknowledgements.
We are grateful to four researchers for supplying fungal strains used in this study: Dr. Jean Loup Notteghem (Center de Coopération Internationale en Recherche Agronomique pour le Devéloppement/CA, Montpellier, France), Dr. Masataka Iwano (Hokuriku Department of Lowland Farming, National Agricultural Research Center, Niigata, Japan), Dr. Hiroshi Yaegashi (Saga University, Saga, Japan), and Dr. Shigehisa Kiyosawa. We also thank Dr. Takashi Kamakura (Laboratory of Chemical Genetics, The Institute of Physical and Chemical Research, Saitama, Japan) for technical advice on the preparation of HMW genomic DNA and for comments on the manuscript; and we thank Dr. Daniel J. Ebbole (Department of Plant Pathology and Microbiology, Texas A&M University, Tex., USA) for kindly giving us permission to use nucleotide sequences of ESTs (mgns001×H03f.b, mgns007×M18f.b).
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Kito, H., Takahashi, Y., Sato, J. et al. Occan, a novel transposon in the Fot1 family, is ubiquitously found in several Magnaporthe grisea isolates. Curr Genet 42, 322–331 (2003). https://doi.org/10.1007/s00294-002-0365-0
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DOI: https://doi.org/10.1007/s00294-002-0365-0