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In vitro bioactivity and biological assays of porous membranes of the poly(lactic acid) containing calcium silicate fibers

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Abstract

A new approach in dentistry is based on the development of resorbable polymeric membranes for guided bone regeneration. The porous membrane surface promotes better cell adhesion and proliferation. Poly(lactic acid) (PLA) is one of most widely used polymers for several biological applications, with the advantage of presenting hydrolysis degradation and bioresorption of its products. In this work, PLA porous polymeric membranes containing calcium silicate (CaSiO3) fibers were prepared by controlled humidity technique. The porous membranes were characterized by scanning electron microscopy, X-ray diffraction, differential scanning calorimetry, bioactivity in simulated body fluid and biological studies in vitro. The incorporation of the CaSiO3 fibers into the polymeric matrix increased the pore size (1.5–34.9 µm). In the bioactivity assay, PLA/CASiO3 membranes induced the formation of a hydroxyapatite layer on the porous membrane surface. Also, in vitro biologic assays showed that the porous membranes provided suitable environment for cell attachment and proliferation.

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Acknowledgements

The authors thank the São Paulo Research Foundation—FAPESP (2015/24659-7)—for financial support and the student Cynthia Guimarães de Paula for the production of the calcium silicate fibers (FAPESP 2012/07897-3).

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Authors and Affiliations

  1. Federal University of São Paulo (UNIFESP), 330 Talim St, São José dos Campos, SP, 12231-280, Brazil

    Idalia Aparecida Waltrick Brito Siqueira, Nayara Koba de Moura, Fábio Roberto Passador & Eliandra Sousa Trichês

  2. Institute of Science and Technology, UNESP – Univ Estadual Paulista, State University of São Paulo (UNESP), 777 Av. Eng. Francisco José Longo, São José dos Campos, SP, 12245000, Brazil

    Suelen Simões Amaral & Luana Marotta Reis de Vasconcellos

  3. National Institute for Space Research (INPE), 1758 dos Astronautas Avenue, São José dos Campos, SP, 12227-010, Brazil

    João Paulo Barros Machado

  4. Federal University of São Carlos, 235 km Washington Luís, São Carlos, SP, 13565-905, Brazil

    Eduardo Henrique Backes

  5. Department of Restorative Dental Sciences, College of Dentistry, University of Florida (UFL), 32611, Gainesville, FL, USA

    Samira Esteves Afonso Camargo

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  1. Idalia Aparecida Waltrick Brito Siqueira
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Correspondence to Idalia Aparecida Waltrick Brito Siqueira.

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Siqueira, I.A.W.B., Amaral, S.S., de Moura, N.K. et al. In vitro bioactivity and biological assays of porous membranes of the poly(lactic acid) containing calcium silicate fibers. Polym. Bull. 77, 5357–5371 (2020). https://doi.org/10.1007/s00289-019-03021-5

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