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Description of Ornithinibacillus massiliensis sp. nov., Isolated from a Child with Marasmus

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Abstract

A Gram-positive, aerobic, motile, endospore-forming, rod-shaped bacterium was isolated from a stool sample of a child with marasmus. The 16S rRNA gene showed that strain Marseille-P3601T exhibited 98.68% sequence identity with Ornithinibacillus scapharcae strain TW25. The genomic DNA G+C contents of this strain was 36.9 mol%. The fatty acid profiles of the strain were iso/anteiso branched structures. The highest DDH value was 20.6%, shared with O. californiensis, amongst its closest strain phylogenetically. Based on the phylogenetic position and the genomic, morphological, and biochemical properties, strain Marseille-P3601T (=CSUR P3601=CCUG 71291) represents a novel species in the genus Ornithinibacillus, for which the name Ornithinibacillus massiliensis sp. nov. is proposed.

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Acknowledgements

We thank Nicholas Armstrong for performing the fatty acid analyses, Amael Fadlane for culturing the strains, Ludivine Brechard for sequencing the genome, and Aurelia Caputo for submitting the genomic sequence to GenBank. We also thank Rita Zgheib for helping in the genomic analysis and Gabriel Haddad for the microscopy photo.We sincerely thank Takashi Irie, Kyoko Imai, Taku Sakazume, Yusuke Ominami, Hisada Akiko and the Hitachi team of Japan (Hitachi High-Tech Corporation, Tokyo, Japan) for the collaborative study conducted together with IHU Méditerranée Infection, and for the installation of a SU5000 microscope at IHU Méditerranée Infection facility.

Funding

This study was supported by the Institut Hospitalo-Universitaire (IHU) Méditerranée Infection, the National Research Agency under the “Investissements d’avenir” programme, reference ANR-10-IAHU-03, the Région Provence Alpes Côte d’Azur and European ERDF PRIMI funding.

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Authors and Affiliations

  1. Aix Marseille University, IRD, AP-HM, MEPHI, Marseille, France

    Rim Iwaza, Ahmad Ibrahim, Thi Phuong Thao Pham, Maryam Tidjani Alou, Didier Raoult & Jean-Christophe Lagier

  2. IHU-Méditerranée Infection, 19-21 Boulevard Jean Moulin, 13385, Marseille cedex 05, France

    Rim Iwaza, Linda Abou Chacra, Ahmad Ibrahim, Thi Phuong Thao Pham, Cheikh Ibrahima Lo, Maryam Tidjani Alou, Cheikh Sokhna, Didier Raoult & Jean-Christophe Lagier

  3. Aix Marseille University, IRD, AP-HM, SSA, VITROME, Marseille, France

    Linda Abou Chacra & Cheikh Ibrahima Lo

  4. Campus Communication, UCAD-IRD of Hann, Dakar, Senegal

    Cheikh Sokhna

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  1. Rim Iwaza
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  2. Linda Abou Chacra
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  4. Thi Phuong Thao Pham
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  8. Didier Raoult
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  9. Jean-Christophe Lagier
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Contributions

Conceptualization, JCL and DR; methodology, JCL; validation, CS, JCL, and DR; formal analysis, RI, LAC, AI, and TP; investigation, RI, TP, CS and JCL; writing-original draft preparation, RI; writing-review and editing, CIL, and MTA supervision, CIL, MTA and JCL; funding acquisition, DR All authors have read and agreed to the published version of the manuscript.

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Correspondence to Jean-Christophe Lagier.

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Iwaza, R., Abou Chacra, L., Ibrahim, A. et al. Description of Ornithinibacillus massiliensis sp. nov., Isolated from a Child with Marasmus. Curr Microbiol 79, 262 (2022). https://doi.org/10.1007/s00284-022-02955-w

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  • DOI: https://doi.org/10.1007/s00284-022-02955-w

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