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Putative Drug Target Identification in Tinea Causing Pathogen Trichophyton rubrum Using Subtractive Proteomics Approach

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Abstract

Trichophyton, important among the three keratinophylic fungi grouped as dermatophytes, is known to cause superficial infections in skin, nail and hair of all the living organisms. The side effects produced by the drugs currently administered to counter these infections have necessitated the search for novel targets. The present study focused on finding putative drug targets in Trichophyton rubrum using the subtractive proteomics approach where its whole proteome was analyzed to find proteins non-homologous to humans inclusive of their gut flora and human protein domain but essential to T. rubrum, to identify sub-cellular localization, functional classification of uncharacterized proteins and to analyze the protein network, druggability and pathway of the targets. The study’s strength relies on its addition of important steps namely, non-homology of the pathogen domain to human domain, non-homology to gut microbiota and substantiation of the importance of the targets in networking by node deletion to the existing methods in drug discovery for dermatophytoses. The study has resulted in the identification of two novel drug targets from the whole proteome of T. rubrum that are not present in human and human gut microbiota.

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Acknowledgements

The authors are thankful to DST-PURSE for financial assistance to carry out this work.

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Authors and Affiliations

  1. Biochematics Laboratory, Department of Bioinformatics, Bharathiar University, Coimbatore, Tamil Nadu, 641 046, India

    Mohamed Hussain Syed Abuthakir, Velusamy Sharmila & Muthusamy Jeyam

  2. Department of Bioinformatics, Bishop Heber College, Tiruchchirappalli, Tamil Nadu, India

    Thomas Jebastin

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  1. Mohamed Hussain Syed Abuthakir
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  2. Thomas Jebastin
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  4. Muthusamy Jeyam
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MJ: Planned and guided the work, SAMH: Executed and written all the works, TJ and SV: Contributed in data analysis and written portions.

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Correspondence to Muthusamy Jeyam.

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Abuthakir, M.H.S., Jebastin, T., Sharmila, V. et al. Putative Drug Target Identification in Tinea Causing Pathogen Trichophyton rubrum Using Subtractive Proteomics Approach. Curr Microbiol 77, 2953–2962 (2020). https://doi.org/10.1007/s00284-020-02114-z

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  • DOI: https://doi.org/10.1007/s00284-020-02114-z

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