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Chryseotalea sanaruensis gen. nov., sp., nov., a Member of the Family Cytophagaceae, Isolated from a Brackish Lake in Hamamatsu Japan

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Abstract

A strain-designated YsT was isolated as a filterable bacterial strain from Lake Sanaru, a brackish water lake in Hamamatsu Japan. YsT is aerobic, Gram-negative, and slender rod shaped. YsT grew optimally at 30 °C, pH 7.0–8.0 and without the addition of NaCl. MK-7 was the sole isoprenoid quinone. The main cellular polar lipids were phosphatidylethanolamine and unidentified amino- and polar-lipids. The predominant cellular fatty acids were C18:0, iso-C14:0 and iso-C15:0. Phylogenetic analysis of 16S rRNA gene sequence revealed the nearest neighbours of strain YsT to be members of the Ohtaekwangia and Chryseolinea genera with 91.2–92.1% sequence similarity. The percentages of conserved proteins (POCP) between the genomes of YsT and related strains were less than 50%. Phenotypic analyses suggested that YsT could not metabolize glucose and related sugars, which was discriminative from its phylogenetic relatives. We, therefore, propose a novel species in a new genus, Chryseotalea sanaruensis gen. nov., sp. nov. in the family Cytophagaceae (= JCM 30318T = LMG 30359T), based on cell size, the predominant cellular fatty acid composition, and the DNA GC content (38.9 mol%).

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Funding

This study was supported by the Institute for Fermentation, Osaka (IFO), Japan.

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Author notes

  1. Tomohiro Suzuki

    Present address: Center for Bioscience Research and Education, Utsunomiya University, 350 Minemachi, Utsunomiya, Tochigi, 321-8505, Japan

  2. Yoshiaki Maejima and Takao Iino have equally contributed to this work.

Authors and Affiliations

  1. Department of Engineering, Graduate School of Integrated Science and Technology, Shizuoka University, 3-5-1 Johoku, Naka-ku, Hamamatsu, Shizuoka, 432-8561, Japan

    Yoshiaki Maejima, Yusuke Muraguchi, Kazuhide Kimbara & Masaki Shintani

  2. Japan Collection of Microorganisms RIKEN, BioResource Research Center, 3-1-1 Koyadai, Tsukuba, Ibaraki, 305-0074, Japan

    Takao Iino, Moriya Ohkuma & Masaki Shintani

  3. Department of Bioscience, Graduate School of Science and Technology, Shizuoka University, 3-5-1 Johoku, Naka-ku, Hamamatsu, Shizuoka, 432-8561, Japan

    Kohei Fukuda & Masaki Shintani

  4. Research Institute of Green Science and Technology, Shizuoka University, 3-5-1 Johoku, Naka-ku, Hamamatsu, Shizuoka, 432-8561, Japan

    Tomohiro Suzuki, Ryota Moriuchi, Hideo Dohra & Masaki Shintani

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  1. Yoshiaki Maejima
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  2. Takao Iino
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Corresponding author

Correspondence to Masaki Shintani.

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Maejima, Y., Iino, T., Muraguchi, Y. et al. Chryseotalea sanaruensis gen. nov., sp., nov., a Member of the Family Cytophagaceae, Isolated from a Brackish Lake in Hamamatsu Japan. Curr Microbiol 77, 306–312 (2020). https://doi.org/10.1007/s00284-019-01823-4

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  • DOI: https://doi.org/10.1007/s00284-019-01823-4

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