Abstract
Dietary proteins can alter gut microbial diversity. However, little is known about how gut bacteria respond to dietary proteins during short-term feeding. In the present study, PCR-DGGE analysis was performed to compare changes in gut microbial composition in rat caecum after rats were fed proteins from soy, pork, beef, chicken, fish and casein (control) for 2, 7 and 14 days. On day 2, differences were observed in microbial composition between groups of red meat (pork and beef) and white meat (chicken and fish) proteins. For a certain diet group, microbial composition showed a great change with feeding time. Principle component analysis indicated that the soy protein group showed a good separation in microbial composition from the casein and meat protein groups on days 7 and 14.
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Acknowledgements
Special thanks were extended to Dr. Yuxiang Yang from Nanjing Agricultural University for his kind help during the experiment.
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This work was funded by the National Natural Science Foundation of China [31471600].
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Supplementary File 1
The DGGE gels of 190 samples from six dietary groups at day 0, day 2, day 7 and day 14 (n = 10 in each group) (RAR 5565 kb)
Supplementary File 2
The band frequency (number of sample existed the band) of six dietary groups at day 0, day 2, day 7 and day 14. (n = 10 in each group) (XLS 31 kb)
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Zhao, F., Huang, Z., Zhou, G. et al. Dietary Proteins Rapidly Altered the Microbial Composition in Rat Caecum. Curr Microbiol 74, 1447–1452 (2017). https://doi.org/10.1007/s00284-017-1339-2
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DOI: https://doi.org/10.1007/s00284-017-1339-2