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UV-C Adaptation of Shigella: Morphological, Outer Membrane Proteins, Secreted Proteins, and Lipopolysaccharides Effects

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Abstract

Water UV disinfection remains extremely important, particularly in developing countries where drinking and reclaimed crop irrigation water may spread devastating infectious diseases. Enteric bacterial pathogens, among which Shigella, are possible contaminants of drinking and bathing water and foods. To study the effect of UV light on Shigella, four strains were exposed to different doses in a laboratory-made irradiation device, given that the ultraviolet radiation degree of inactivation is directly related to the UV dose applied to water. Our results showed that the UV-C rays are effective against all the tested Shigella strains. However, UV-C doses appeared as determinant factors for Shigella eradication. On the other hand, Shigella-survived strains changed their outer membrane protein profiles, secreted proteins, and lipopolysaccharides. Also, as shown by electron microscopy transmission, morphological alterations were manifested by an internal cytoplasm disorganized and membrane envelope breaks. Taken together, the focus of interest of our study is to know the adaptive mechanism of UV-C resistance of Shigella strains.

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Acknowledgements

The authors would like to thank the entire team of electron microscopy of the Murcia University for their help, and gratefully acknowledge the support of the microbiology research unit of the Autonomous University of Barcelona.

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Authors and Affiliations

  1. Laboratory of Wastewater Treatment and Valorization, Water Research and Technology Centre, Technopole of Borj-Cédria, BP 273, Soliman, 8020, Tunisia

    Kalthoum Chourabi, Salma Kloula & Abdelwaheb Chatti

  2. Department of Genetics and Microbiology, Autonomous University of Barcelona, 08290, Barcelona, Spain

    Susana Campoy & Jesus A. Rodriguez

  3. Laboratory of Biochemistry and Molecular Biology, Faculty of Sciences of Bizerte, Carthage University, Zarzouna, 7021, Tunisia

    Ahmed Landoulsi

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  1. Kalthoum Chourabi
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  2. Susana Campoy
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Correspondence to Kalthoum Chourabi.

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Chourabi, K., Campoy, S., Rodriguez, J.A. et al. UV-C Adaptation of Shigella: Morphological, Outer Membrane Proteins, Secreted Proteins, and Lipopolysaccharides Effects. Curr Microbiol 74, 1261–1269 (2017). https://doi.org/10.1007/s00284-017-1311-1

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