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Use of Vine-Trimming Wastes as Carrier for Amycolatopsis sp. to Produce Vanillin, Vanillyl Alcohol, and Vanillic Acid

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Abstract

Raw vine-trimming wastes or the solid residues obtained after different fractionation treatments were evaluated for their suitability as Amycolatopsis sp. immobilization carriers during the bioconversion of ferulic acid into valuable phenolic compounds such as vanillin, vanillyl alcohol, and vanillic acid, the main flavor components of vanilla pods. Previously, physical–chemical characteristics of the materials were determined by quantitative acid hydrolysis and water absorption index (WAI), and microbiological characteristics by calculating the cell retention in the carrier (λ). Additionally, micrographics of carrier surface were obtained by field emission-scanning electron microscopy to study the influence of morphological changes during pretreatments in the adhesion of cells immobilized. The results point out that in spite of showing the lowest WAI and intermediate λ, raw material was the most appropriated substrate to conduct the bioconversion, achieving up to 262.9 mg/L phenolic compounds after 24 h, corresponding to 42.9 mg/L vanillin, 115.6 mg/L vanillyl alcohol, and 104.4 mg/L vanillic acid. The results showed the potential of this process to be applied for biotechnological production of vanillin from ferulic acid solutions; however, further studies must be carried out to increase vanillin yield. Additionally, the liquors obtained after treatment of vine-trimming wastes could be assayed to replace synthetic ferulic acid.

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Acknowledgments

We thank the Spanish Ministry of Economy and Competitiveness for the financial support of this work (project CTQ2015-71436-C2-1-R), which has partial financial support from the FEDER funds of the European Union; the Spanish Ministry of Education, Culture and Sports for Pérez-Rodríguez‘s FPU; and CAPES (Coordination of High-Level Personnel Training, Brazil) for Prof. J. M. Domínguez fellowship as Special Visiting Researcher (Science without Borders Program, project n. 88881.062223/2014-01). Authors also thank the Mexican National Council for Science and Technology (CONACYT) for financial support to M.Sc. Juan Francisco Castañón Rodríguez for his research stay in Spain.

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Authors and Affiliations

  1. Department of Chemical Engineering, Faculty of Sciences, University of Vigo (Campus Ourense), As Lagoas s/n, 32004, Ourense, Spain

    Juan Francisco Castañón-Rodríguez, Noelia Pérez-Rodríguez & José Manuel Domínguez

  2. Laboratory of Agro-food Biotechnology, CITI (University of Vigo)-Tecnópole, Parque Tecnológico de Galicia, San Cibrao das Viñas, 32900, Ourense, Spain

    Juan Francisco Castañón-Rodríguez, Noelia Pérez-Rodríguez & José Manuel Domínguez

  3. Instituto Tecnológico de Veracruz-UNIDA, Av. Miguel A. de Quevedo 2779, Col. Formando Hogar, 91860, Veracruz, VER, Mexico

    Juan Francisco Castañón-Rodríguez & María Guadalupe Aguilar-Uscanga

  4. Department of Biochemical and Pharmaceutical Technology, Faculty of Pharmaceutical Sciences, University of São Paulo, Av. Lineu Prestes 580, Bl 16, São Paulo, 05508-900, Brazil

    Ricardo Pinheiro de Souza Oliveira

Authors
  1. Juan Francisco Castañón-Rodríguez
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  2. Noelia Pérez-Rodríguez
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  3. Ricardo Pinheiro de Souza Oliveira
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  4. María Guadalupe Aguilar-Uscanga
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  5. José Manuel Domínguez
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Correspondence to José Manuel Domínguez.

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Castañón-Rodríguez, J.F., Pérez-Rodríguez, N., de Souza Oliveira, R.P. et al. Use of Vine-Trimming Wastes as Carrier for Amycolatopsis sp. to Produce Vanillin, Vanillyl Alcohol, and Vanillic Acid. Curr Microbiol 73, 561–568 (2016). https://doi.org/10.1007/s00284-016-1094-9

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  • DOI: https://doi.org/10.1007/s00284-016-1094-9

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