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Lysobacter chengduensis sp. nov. Isolated from the Air of Captive Ailuropoda melanoleuca Enclosures in Chengdu, China

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Abstract

A novel bacterial strain, designated as CF21T, was isolated from the air of Ailuropoda melanoleuca enclosures in China. Cells were gram-negative, aerobic, non-motile, and rod shaped. Strain CF21T grew at 10–40 °C (optimum 28–30 °C) and pH 6.0–9.0 (optimum pH 7.0–8.0) and in the presence of NaCl concentrations ranging from 0.0 % (w/v) to 2.0  % (optimum 0.0–1.0 %). 16SrRNA gene sequence analysis indicated that strain CF21T belonged to genus Lysobacter within class Gammaproteobacteria and was most closely related to Luteimonas dalianensi OB44-3T (95.8 % similarity), Lysobacter ruishenii CTN-1T (95.1 %), Lysobacter spongiicola KMM329T (94.8 %), and Lysobacter daejeonensis GH1-9T (94.6 %). The genomic G+C DNA content was 68.72 mol%. Major cellular fatty acids of CF21T were iso-C16:0 (30.22 %), iso-C15:0 (25.70 %), and the sum of 10-methyl C16 : 0 and/or iso-C17 : 1ω9c (21.94 %). The prominent isoprenoid quinone was ubiquinone 8 (Q-8). Primary polar lipids included diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, and an unknown phospholipid. DNA sequence relatedness between strain CF21T and L. ruishenii CTN-1T was 56 %, which was clearly below the 70 % threshold for prokaryotic species delineation. These analyses indicated that CF21T is a novel member of genus Lysobacter, for which the name Lysobacter chengduensis sp. nov. is proposed. The type strain is CF21T (=CGMCC1.15145T = DSM 100306T).

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Acknowledgments

We are grateful to Dr. Jian-Dong Jiang of Nanjing Agricultural University for providing L. ruishenii CTN-1T and L. daejeonensis GH1-9T. This work was supported by the National Science and Technology Support Project (2012BAC01B06) and Chengdu Giant Panda Breeding Research Foundation (CPF2010-06), China.

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Authors and Affiliations

  1. College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, 611130, China

    Caifang Wen, Lixin Xi, Rong She, Shan Zhao, Zhongxiang Hao, Lu Luo, Hong Liao, Zhenrong Chen, Sanjie Cao, Rui Wu & Qigui Yan

  2. College of Food Science, Sichuan Agricultural University, Yaan, China

    Guoquan Han

  3. Chengdu Research Base of Giant Panda Breeding, Chengdu, China

    Rong Hou

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  1. Caifang Wen
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Correspondence to Qigui Yan.

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284_2015_921_MOESM1_ESM.docx

Polar lipids analyses of strain Lysobacter chengduensis CF21T were carried out using a two-dimensional TLC method. PE, Phosphatidylethanolamine; PG, phosphatidylglycerol; DPG, diphosphatidylglycerol, and UPL, an unknown phospholipid. Supplementary material 1 (DOCX 612 kb)

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Wen, C., Xi, L., She, R. et al. Lysobacter chengduensis sp. nov. Isolated from the Air of Captive Ailuropoda melanoleuca Enclosures in Chengdu, China. Curr Microbiol 72, 88–93 (2016). https://doi.org/10.1007/s00284-015-0921-8

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