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Carbon and Nitrogen Sources Influence Tricalcium Phosphate Solubilization and Extracellular Phosphatase Activity by Talaromyces flavus

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Abstract

The aim of this work was to study phosphate (P) solubilization (and the processes involved in this event) by Talaromyces flavus (BAFC 3125) as a function of carbon and/or nitrogen sources. P solubilization was evaluated in NBRIP media supplemented with different carbon (glucose, sorbitol, sucrose, and fructose) and nitrogen (l-asparagine, urea, ammonium sulfate (AS), and ammonium nitrate (AN) combinations. The highest P solubilization was related to the highest organic acid production (especially gluconic acid) and pH drop for those treatments where glucose was present. Also P solubilization was higher when an inorganic nitrogen source was supplemented to the media when compared to an organic one. Although not being present an organic P source, phosphatase activity was observed. This shows that P mineralization and P solubilization can occur simultaneously, and that P mineralization is not induced by the enzyme substrate. The combination that showed highest P solubilization was for AN-glucose. The highest acid phosphatase activity was for AS-fructose, while for alkaline phosphatase were for AS-fructose and AN-fructose. Acid phosphatase activity was higher than alkaline. P solubilization and phosphatase activity (acid and alkaline) were influenced by the different carbon–nitrogen combinations. A better understanding of phosphate-solubilizing fungi could bring a better use of soil P.

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Acknowledgments

This work was supported by the following institutions: Universidad de Buenos Aires (UBA), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), and Agencia Nacional de Promoción Científica y Tecnológica (ANPCyT).

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    Authors and Affiliations

    1. Departamento de Biodiversidad y Biología Experimental, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Pabellón II, 4th floor Ciudad Universitaria, 1428, Buenos Aires, Argentina

      P. J. Stefanoni Rubio, I. F. Della Mónica & A. M. Godeas

    2. Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Pabellón II, 4th floor Ciudad Universitaria, 1428, Buenos Aires, Argentina

      M. S. Godoy & M. J. Pettinari

    3. Departamento de Botánica, INIBIOMA (CONICET-UNComahue), Quintral 1250, 8400, San Carlos de Bariloche, Río Negro, Argentina

      J. M. Scervino

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    1. P. J. Stefanoni Rubio
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    2. M. S. Godoy
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    3. I. F. Della Mónica
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    4. M. J. Pettinari
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    5. A. M. Godeas
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    6. J. M. Scervino
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    Correspondence to J. M. Scervino.

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    P. J. Stefanoni Rubio and M. S. Godoy have contributed equally to this work.

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    Stefanoni Rubio, P.J., Godoy, M.S., Della Mónica, I.F. et al. Carbon and Nitrogen Sources Influence Tricalcium Phosphate Solubilization and Extracellular Phosphatase Activity by Talaromyces flavus . Curr Microbiol 72, 41–47 (2016). https://doi.org/10.1007/s00284-015-0914-7

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    • DOI: https://doi.org/10.1007/s00284-015-0914-7

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