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Phenotypic and Molecular Characterization of Leptospira interrogans Isolated from Canis familiaris in Southern Brazil

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Abstract

Leptospirosis is a zoonotic disease caused by pathogenic spirochetes from the genus Leptospira, which includes 20 species and more than 300 serovars. Canines are important hosts of pathogenic leptospires and can transmit the pathogen to humans via infected urine. Here, we report the phenotypic and molecular characterization of Leptospira interrogans isolated from Canis familiaris in Southern Brazil. The isolated strain was characterized by variable-number tandem-repeats analysis as L. interrogans, serogroup Icterohaemorrhagiae. In addition, the isolate was recognized by antibodies from human and canine serum samples previously tested by microscopic agglutination test. Ultimately, the expression of membrane-associated antigens (LipL32 and leptospiral immunoglobulin-like proteins) from pathogenic leptospires using monoclonal antibodies was detected by indirect immunofluorescence assay. In conclusion, identification of new strains of Leptospira can help in the diagnosis and control of leptospirosis.

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Acknowledgments

The authors are grateful to Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) and Fundação de Amparo à Pesquisa do Estado do Rio Grande do Sul (FAPERGS).

Conflict of interest

The authors certify that they have no potential conflicts of interest.

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Author notes

    Authors and Affiliations

    1. Centro de Desenvolvimento Tecnológico, Núcleo de Biotecnologia, Laboratório de Vacinologia, Universidade Federal de Pelotas, Campus Universitário Capão do Leão, CP 354, Pelotas, RS, CEP 96010-900, Brazil

      Sérgio Jorge, Natasha R. De Oliveira & Odir A. Dellagostin

    2. Centro de Desenvolvimento Tecnológico, Núcleo de Biotecnologia, Laboratório de Imunodiagnóstico, Universidade Federal de Pelotas, Campus Universitário Capão do Leão, CP 354, Pelotas, RS, CEP 96010-900, Brazil

      Leonardo G. Monte, Thais F. Collares, Bárbara C. Roloff, Charles K. Gomes & Cláudia P. Hartleben

    3. Centro de Desenvolvimento Tecnológico, Núcleo de Biotecnologia, Instituto de Biologia, Departamento de Microbiologia e Parasitologia, Laboratório de Bacteriologia, Universidade Federal de Pelotas, Campus Universitário Capão do Leão, CP 354, Pelotas, RS, CEP 96010-900, Brazil

      Daiane D. Hartwig

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    1. Sérgio Jorge
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    2. Leonardo G. Monte
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    3. Natasha R. De Oliveira
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    4. Thais F. Collares
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    5. Bárbara C. Roloff
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    6. Charles K. Gomes
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    7. Daiane D. Hartwig
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    8. Odir A. Dellagostin
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    9. Cláudia P. Hartleben
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    Corresponding author

    Correspondence to Cláudia P. Hartleben.

    Additional information

    Sérgio Jorge and Leonardo G. Monte have contributed equally to the experimental work.

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    Jorge, S., Monte, L.G., De Oliveira, N.R. et al. Phenotypic and Molecular Characterization of Leptospira interrogans Isolated from Canis familiaris in Southern Brazil. Curr Microbiol 71, 496–500 (2015). https://doi.org/10.1007/s00284-015-0857-z

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    • DOI: https://doi.org/10.1007/s00284-015-0857-z

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