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Characterization and Genomic Analysis of Quinolone-Resistant Delftia sp. 670 Isolated from a Patient Who Died from Severe Pneumonia

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Abstract

Antibiotic-resistant opportunistic pathogens have become a serious concern in recent decades, as they are increasingly responsible for hospital-acquired infections. Here, we describe quinolone-resistant Delftia sp. strain 670, isolated from the sputum of a patient who died from severe pulmonary infection. The draft genome sequence of this strain was obtained by whole-genome shotgun sequencing, and was subjected to comparative genome analysis. Genome analysis revealed that one critical mutation (Ser83Ile in gyrA) might play a decisive role in quinolone resistance. The genome of Delftia sp. strain 670 contains both type II and type VI secretion systems, which were predicted to contribute to the virulence of the strain. Phylogenetic analysis, assimilation tests, and comparative genome analysis indicated that strain 670 differed from the four known Delftia species, suggesting this strain could represent a novel species. Although the study could not determine the strain 670 as the pathogen led to mortality, our findings also presented the pathogenic potential of Delftia species, and the increasing severity of antibiotic resistance among emerging opportunistic pathogens. The whole genome sequencing and comparative analysis improved our understanding of genome evolution in the genus Delftia, and provides the foundation for further study on drug resistance and virulence of Delftia strains.

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Acknowledgments

This research was supported by a grant from the National Hi-Tech Research and Development (863) Program of China (No. 2012AA022003 and No.2014AA021402), the China Mega-Project on Infectious Disease Prevention (Nos. 2013ZX10004605, 2011ZX10004001, 2013ZX10004607-004 and 2013ZX10004217-002-003) and the State Key Laboratory of Pathogen and BioSecurity Program (No. SKLPBS1113).

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The authors declare that they have no conflict interest.

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    Authors and Affiliations

    1. School of Basic Medical Science Central South University, Changsha, 410013, People’s Republic of China

      Huaixing Kang

    2. Anhui Medical University, Hefei, 230032, People’s Republic of China

      Xiaomeng Xu

    3. State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing, 100071, People’s Republic of China

      Huaixing Kang, Xiaomeng Xu, Xioaping An, Zhiqiang Mi, Fan Peng, Guangqian Pei, Yahui Wang, Yong Huang, Xianglilan Zhang, Zhiyi Zhang, Wei Wang & Yigang Tong

    4. Affiliated Hospital, Academy of Military Medical Sciences, Beijing, 100071, People’s Republic of China

      Xiuyun Yin & Jiankui Chen

    5. Central Laboratory, Navy General Hospital, Beijing, People’s Republic of China

      Kaifei Fu & Lijun Zhou

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    Correspondence to Lijun Zhou, Jiankui Chen or Yigang Tong.

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    Huaixing Kang, Xiaomeng Xu and Kaifei Fu have contributed equally to this work.

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    Kang, H., Xu, X., Fu, K. et al. Characterization and Genomic Analysis of Quinolone-Resistant Delftia sp. 670 Isolated from a Patient Who Died from Severe Pneumonia. Curr Microbiol 71, 54–61 (2015). https://doi.org/10.1007/s00284-015-0818-6

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