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Genome Sequence and Phenotypic Characterization of Caulobacter segnis

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Abstract

Caulobacter segnis is a unique species of Caulobacter that was initially deemed Mycoplana segnis because it was isolated from soil and appeared to share a number of features with other Mycoplana. After a 16S rDNA analysis showed that it was closely related to Caulobacter crescentus, it was reclassified C. segnis. Because the C. segnis genome sequence available in GenBank contained 126 pseudogenes, we compared the original sequencing data to the GenBank sequence and determined that many of the pseudogenes were due to sequence errors in the GenBank sequence. Consequently, we used multiple approaches to correct and reannotate the C. segnis genome sequence. In total, we deleted 247 bp, added 14 bp, and changed 8 bp resulting in 233 fewer bases in our corrected sequence. The corrected sequence contains only 15 pseudogenes compared to 126 in the original annotation. Furthermore, we found that unlike Mycoplana, C. segnis divides by fission, producing swarmer cells that have a single, polar flagellum.

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Acknowledgments

The authors wish to thank the DOE Joint Genome Institute for providing the original sequencing data for the C. segnis genome, and Dr. Yves Brun for providing a culture of C. segnis TK0059 and for submitting the corrected genome sequence and annotation to NCBI. We also thank Dr. Aretha Fiebig for helpful discussions about holdfast genes.

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  1. Department of Biological Sciences, University of South Carolina, Columbia, SC, 29208, USA

    Sagar Patel, Brock Fletcher, Derrick C. Scott & Bert Ely

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  1. Sagar Patel
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  2. Brock Fletcher
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  4. Bert Ely
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Correspondence to Bert Ely.

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Patel, S., Fletcher, B., Scott, D.C. et al. Genome Sequence and Phenotypic Characterization of Caulobacter segnis . Curr Microbiol 70, 355–363 (2015). https://doi.org/10.1007/s00284-014-0726-1

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