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Microbispora sp. LGMB259 Endophytic Actinomycete Isolated from Vochysia divergens (Pantanal, Brazil) Producing β-Carbolines and Indoles with Biological Activity

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Abstract

Endophytic actinomycetes encompass bacterial groups that are well known for the production of a diverse range of secondary metabolites. Vochysia divergens is a medicinal plant, common in the “Pantanal” region (Brazil) and was focus of many investigations, but never regarding its community of endophytic symbionts. During a screening program, an endophytic strain isolated from the V. divergens, was investigated for its potential to show biological activity. The strain was characterized as Microbispora sp. LGMB259 by spore morphology and molecular analyze using nucleotide sequence of the 16S rRNA gene. Strain LGMB259 was cultivated in R5A medium producing metabolites with significant antibacterial activity. The strain produced 4 chemically related β-carbolines, and 3 Indoles. Compound 1-vinyl-β-carboline-3-carboxylic acid displayed potent activity against the Gram-positive bacterial strains Micrococcus luteus NRRL B-2618 and Kocuria rosea B-1106, and was highly active against two human cancer cell lines, namely the prostate cancer cell line PC3 and the non-small-cell lung carcinoma cell line A549, with IC50 values of 9.45 and 24.67 µM, respectively. 1-Vinyl-β-carboline-3-carboxylic acid also showed moderate activity against the yeast Saccharomyces cerevisiae ATCC204508, as well as the phytopathogenic fungi Phyllosticta citricarpa LGMB06 and Colletotrichum gloeosporioides FDC83.

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Acknowledgments

We thank Dr. Jack Goodman (University of Kentucky) for the HRESIMS measurements and Dr. Francisco André Ossamu Tanaka (NAP/MEPA) for the Scanning Electron Microscope analysis. D. C. S. thanks the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) for a short-term scholarship. This work was supported in part by the University of Kentucky College of Pharmacy, the University of Kentucky Markey Cancer Center and the National Center for Advancing Translational Sciences (UL1TR000117). This work was also supported by NIH grants CA 091901 and GM 105977 to J.R., and Fundação Araucária—Apoio ao desenvolvimento científico e tecnológico do Paraná.

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Authors and Affiliations

  1. Department of Pharmaceutical Sciences, College of Pharmacy, University of Kentucky, Lexington, KY, 40536-0596, USA

    Daiani C. Savi, Khaled A. Shaaban, Larissa V. Ponomareva, Jon S. Thorson & Jürgen Rohr

  2. Department of Microbiology, Parasitology and Pathology, Universidade Federal do Parana, Av. Coronel Francisco Heráclito dos Santos, 210, Curitiba, PR, CEP 81531-970, Brazil

    Daiani C. Savi

  3. Center for Pharmaceutical Research and Innovation (CPRI), College of Pharmacy, University of Kentucky, Lexington, KY, 40536-0596, USA

    Khaled A. Shaaban, Larissa V. Ponomareva & Jon S. Thorson

  4. Department of Genetics, Universidade Federal do Parana, PO. BOX 19071, Curitiba, PR, CEP 81531-970, Brazil

    Nathalia Vargas & Chirlei Glienke

  5. Department of Biology, Universidade Federal do Mato Grosso do Sul, Campo Grande, MS, CEP 79070-900, Brazil

    Yvelise M. Possiede

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  1. Daiani C. Savi
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  2. Khaled A. Shaaban
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Corresponding authors

Correspondence to Chirlei Glienke or Jürgen Rohr.

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Daiani C. Savi and Khaled A. Shaaban have contributed equally to this work.

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Savi, D.C., Shaaban, K.A., Vargas, N. et al. Microbispora sp. LGMB259 Endophytic Actinomycete Isolated from Vochysia divergens (Pantanal, Brazil) Producing β-Carbolines and Indoles with Biological Activity. Curr Microbiol 70, 345–354 (2015). https://doi.org/10.1007/s00284-014-0724-3

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  • DOI: https://doi.org/10.1007/s00284-014-0724-3

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