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Diversity of Lactic Acid Bacteria Associated with Fresh Coffee Cherries in Taiwan

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Abstract

A total of 102 lactic acid bacteria (LAB) were isolated from three different coffee farms in Taiwan. These isolates were classified and identified by the restriction fragment length polymorphism analysis and sequencing of 16S ribosomal DNA. Heterofermentative Leuconostoc, and Weissella species were the most common LAB found in two farms located at an approximate altitude of 800 m. Lactococcus lactis subsp. lactis was the most common LAB found in the remaining farm was located at an approximate altitude of 1,200 m. It is therefore suggested that the altitude and climate may affect the distribution of LAB. On the basis of phylogenetic analysis, two strains included in the genera Enterococcus were considered as two potential novel species or subspecies. In addition, a total of 34 isolates showed the antifungal activity against Aspergillus flavus. Moreover, seven Lactococcus lactis subsp. lactis strains and one Enterococcus faecalis strain were found to have bacteriocin-like inhibitory substance-producing capability. These results suggest that various LAB are associated with fresh coffee cherries in Taiwan. Some of the isolates found in this study showed potential as antifungal agents.

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Acknowledgments

The authors would like to thank the National Science Council Taiwan for financially supporting this study, under Contract No. NSC 101-2313-B-130-001 granted to Shwu-fen Pan, and Contract No. NSC 102-2313-B-130-001 granted to Yi-sheng Chen.

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Authors and Affiliations

  1. Department of Biotechnology, Ming Chuan University, No. 5 De-Ming Road, Gui-Shan Township, Taoyuan, 333, Taiwan

    Kun-hon Leong, Yi-sheng Chen, Shwu-fen Pan, Jen-jye Chen, Hui-chung Wu & Yu-chung Chang

  2. The Institute of Enology and Viticulture, Yamanashi University, 1-13-1 Kitashin, Kofu, Yamanashi, 400-0005, Japan

    Fujitoshi Yanagida

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  1. Kun-hon Leong
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  2. Yi-sheng Chen
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  3. Shwu-fen Pan
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  4. Jen-jye Chen
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  5. Hui-chung Wu
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  6. Yu-chung Chang
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  7. Fujitoshi Yanagida
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Correspondence to Yi-sheng Chen.

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Kun-hon Leong and Yi-sheng Chen have contributed equally to this work.

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Leong, Kh., Chen, Ys., Pan, Sf. et al. Diversity of Lactic Acid Bacteria Associated with Fresh Coffee Cherries in Taiwan. Curr Microbiol 68, 440–447 (2014). https://doi.org/10.1007/s00284-013-0495-2

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  • DOI: https://doi.org/10.1007/s00284-013-0495-2

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