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Chitinolyticbacter meiyuanensis SYBC-H1T, Gen. Nov., sp. Nov., a Chitin-Degrading Bacterium Isolated From Soil

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Abstract

A novel aerobic mesophilic bacterial strain SYBC-H1T capable of degrading chitin was isolated and classified in this study. The strain exhibited strong chitinolytic activity and was a Gram-negative, curved, rod-shaped, and motile bacterium. Growth of this strain was observed between 10 and 41°C and between pH 3.5 and 9.5. The DNA G + C content of strain SYBC-H1T was 53.25 mol%. The cellular fatty acids (>5%) were 12:0 iso 3-OH (5.87%), 16:0 (28.16%), and 18:1ω7c (20.48%). Phylogenetic analysis based on 16S rRNA gene sequence similarity revealed that strain SYBC-H1T belonged to the family Neisseriaceae, and was distantly related (95.0% similarity) to the genus Chitiniphilus. Its phenotype was unique and genetic and phylogenetic analysis experiments suggested that strain SYBC-H1T represented the type strain (CGMCC 3438T, ATCC BAA-2140T) of a novel genus, for which the name Chitinolyticbacter meiyuanensis SYBC-H1T gen. nov., sp. nov. was proposed. The highest enzymatic activity of chitinase (9.6 U/ml) was obtained at 72 h in 250 ml shake flasks. The 16S rRNA gene sequence of SYBC-H1T has been deposited in GenBank under the accession number GQ981314.

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Acknowledgments

This study was financially supported by the National High Technology and Development Program of China (863 Program, Grant no. 2010AA101501) and National Institutes of Health grant GM-33048.

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Authors and Affiliations

  1. The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, 214122, People’s Republic of China

    Zhikui Hao, Yujie Cai, Xiangru Liao, Jiayang Liu & Mingming Hu

  2. Fishery Extension Station of Yingzhou District, 28 Yingzhou Road, Fuyang, 236000, Anhui, People’s Republic of China

    Zhikui Hao

  3. Energy Research Institute of Shandong Academy of Sciences, Jinan, 250014, People’s Republic of China

    Xiaohui Liang

  4. Department of Cell Biology and Cell Dynamics Program, University of Massachusetts Medical School, Worcester, MA, 01605, USA

    Zhiyou Fang

  5. Jiangsu Hanbon Science & Technology Co. Ltd, Huai’an, 223001, Jiangsu, People’s Republic of China

    Dabing Zhang

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  1. Zhikui Hao
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Correspondence to Xiangru Liao.

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Hao, Z., Cai, Y., Liao, X. et al. Chitinolyticbacter meiyuanensis SYBC-H1T, Gen. Nov., sp. Nov., a Chitin-Degrading Bacterium Isolated From Soil. Curr Microbiol 62, 1732–1738 (2011). https://doi.org/10.1007/s00284-011-9921-5

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