Abstract
The Gluconobacter oxydans M5 with disruption of the pyrroloquinoline quinine-dependent membrane-bound aldehyde dehydrogenase (ALDH) was used for the oxidation of benzyl alcohol. The selectivity toward benzaldehyde showed an obvious increase for the engineered strain, which reached the 67.3%, while the wild strain had only 2.8%. Meantime, the aqueous/isooctane (1:1) biphasic system was used for the further improvement of selectivity. By these methods, nearly 100% selectivity and conversion rate could be obtained within 1 h at the optimum initial benzyl alcohol concentration of 5.0 g/l.
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Acknowledgments
This work was financially supported by the National Key Basic Research Development Program of China (“973” Program, No.2009CB724703), and the National Special Fund for State Key Laboratory of Bioreactor Engineering, Grant No. 2060204.
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Wu, J., Li, M.H., Lin, J.P. et al. Highly Selective Oxidation of Benzyl Alcohol Using Engineered Gluconobacter Oxydans in Biphasic System. Curr Microbiol 62, 1123–1127 (2011). https://doi.org/10.1007/s00284-010-9831-y
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DOI: https://doi.org/10.1007/s00284-010-9831-y