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Identification of Two Species of Yeast-like Symbiotes in the Brown Planthopper, Nilaparvata lugens

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Abstract

To determine the species of the yeast-like symbionts (YLS) in the brown planthoppers (BPH), Nilaparvata lugens, YLS were first isolated and purified by ultracentrifugation from the fat bodies of BPH, and then 18S rDNA and internal transcribed spacer (ITS)–5.8S rDNA sequences of YLS were amplified with the different general primers for fungi. The results showed that the two different 18S and ITS–5.8S rDNA sequences of YLS were obtained. One 2291-bp DNA sequence, which contained 18S and ITS–5.8S rDNA, showed the high similarity to Cryptococcus and was named Cryp-Like symbiotes. Another 1248-bp DNA sequence, which contained a part of 18S and ITS–5.8S rDNA, showed the high similarity to Pichia guilliermondii and was named Pichia-Like symbiotes. It was further proved that Cryp- and Pichia-Like symbiotes existed in BPH through nested PCR with specific primers for two symbiotes and in situ hybridization analysis using digoxigenin-labeled probes. Our results showed that BPH harbored more than one species of eukaryotic YLS, which suggested that diversity of fungal endosymbiotes may be occurred in planthoppers, just like bacterial endosymbiotes.

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Acknowledgments

This work was partly supported by National Key Technology R&D Program in the 11th Five year Plan of China (Grant No. 2008BADA5B06-1), Zhejiang Provincial Natural Science Foundation of China (Grant No. Y3080031), International Foundation for Science (IFS) (Grant No. C/4689-1) and National Natural Science Foundation of China (Grant No. 30900944).

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Authors and Affiliations

  1. Zhejiang Provincial Key Laboratory of Biometrology and Inspection & Quarantine, China Jiliang University, Hangzhou, Zhejiang, 310018, China

    ShengZhang Dong, Kun Pang, Xu Bai, XiaoPing Yu & PeiYing Hao

  2. Xueyuan Street, Xiasha, Hangzhou, Zhejiang, 310018, China

    XiaoPing Yu

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  1. ShengZhang Dong
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  2. Kun Pang
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  3. Xu Bai
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Correspondence to XiaoPing Yu.

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Dong, S., Pang, K., Bai, X. et al. Identification of Two Species of Yeast-like Symbiotes in the Brown Planthopper, Nilaparvata lugens . Curr Microbiol 62, 1133–1138 (2011). https://doi.org/10.1007/s00284-010-9830-z

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