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Adhesion Ability of Campylobacter jejuni to Ht-29 Cells Increases with the Augmentation of Oxidant Agent Concentration

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Abstract

Campylobacter jejuni (C. jejuni) is a leading cause of human enteritis worldwide and the most frequently reported zoonotic agent in the European Union. Despite the fact that C. jejuni is a microaerobic bacteria, known as a fragile one, it is able to survive through adverse conditions such as oxidative stress. The purpose of this study was first to test the oxidative stress resistance in 22 C. jejuni strains of various origins, and to compare adhesive and invasive abilities of four selected strains in the intestinal cell line Ht-29. Secondly, the effect of an oxidative stress on C. jejuni adhesion to Ht-29 cells was investigated. Results show that all the tested strains were able to survive after a 24-h incubation period in broth containing 10 μM of paraquat. From 12.5 μM of paraquat, bacterial strains exhibit different behaviour, and only three strains are able to survive at 25 μM of paraquat. In addition, this study revealed that the number of bound bacteria to epithelial cells increases with augmentation of paraquat concentration, suggesting a link between oxidative stress survival of C. jejuni and virulence on Ht-29 cells.

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Acknowledgements

We are grateful to Magalie Ritz for her scientific help. We thank the Microbiological Laboratory of Public Health of the National Institute for Public Health and the Environment (The Netherlands) and Agence Française de Sécurité Sanitaire des Aliments (AFSSA, Unité HQPAP, Ploufragan, France) for providing C. jejuni strains. This study was supported by grants from the Institut National de la Recherche Agronomique (INRA), and from the Région des Pays de la Loire.

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Authors and Affiliations

  1. UMR-INRA 1014 SECALIM ONIRIS, Site de la Chantrerie, Route de Gachet–La Chantrerie, BP 40706, 44307, Nantes Cedex 03, France

    Nabila Haddad, Gaëlle Maillart, Florence Jugiau, Michel Federighi & Jean-Michel Cappelier

  2. IGM, Batiment 400, Université Paris-Sud, 91400, Orsay, France

    Amélie Garénaux

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  1. Nabila Haddad
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  2. Gaëlle Maillart
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  3. Amélie Garénaux
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  4. Florence Jugiau
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  5. Michel Federighi
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  6. Jean-Michel Cappelier
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Correspondence to Jean-Michel Cappelier.

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Haddad, N., Maillart, G., Garénaux, A. et al. Adhesion Ability of Campylobacter jejuni to Ht-29 Cells Increases with the Augmentation of Oxidant Agent Concentration. Curr Microbiol 61, 500–505 (2010). https://doi.org/10.1007/s00284-010-9644-z

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  • DOI: https://doi.org/10.1007/s00284-010-9644-z

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