Abstract
The light-harvesting antenna (LH) proteins of Rhodospirillum rubrum are encoded by pufA and pufB with C-terminal extensions not present in the mature proteins. Point mutations were introduced into pufB, pufA, and both pufB and pufA so that proteins equivalent to the mature proteins were encoded. The mutants with these truncated proteins produced 3–30% of the wild-type level of LH. These findings suggest that the C-terminal extensions are required for wild-type levels of LH.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Anbudurai PR, Mor TS, Ohad I et al (1994) The ctpA gene encodes the C-terminal processing protease for the D1 protein of the photosystem II reaction center complex. Proc Nat Acad Sci USA 91:8082–8086
Bélanger G, Gingras G (1988) Structure and expression of the puf operon messenger RNA in Rhodospirillum rubrum. J Biol Chem 263:7639–7645
Benson JA (2007) Production and assembly of light-harvesting antenna in Rhodospirillum rubrum. MS thesis, University of Wisconsin-Milwaukee
Bérard J, Bélanger G, Corriveau P et al (1986) Molecular cloning and sequence of the B880 holochrome gene from Rhodospirillum rubrum. J Biol Chem 261:82–87
Cogdell RJ, Hawthornwaite AM, Ferguson LA et al (1990) The structure and function of some unusual variable antenna complexes. In: Drews G, Dawes EA (eds) Molecular biology of membrane-bound complexes in phototrophic bacteria. Plenum, New York, pp 211–217
Crook SM, Treml SB, Collins MLP (1986) Immunocytochemical ultrastructural analysis of chromatophore membrane formation in Rhodospirillum rubrum. J Bacteriol 167:89–95
Dehio C, Meyer M (1997) Maintenance of broad-host-range incompatibility group P and group Q plasmids and transposition of Tn5 in Bartonella henselae following conjugal plasmid transfer from Escherichia coli. J Bacteriol 179:538–540
Hagemann GE, Katsiou E, Forkl H et al (1997) Gene cloning and regulation of gene expression of the puc operon from Rhodovulum sulfidophilum. Biochim Biophys Acta 1351:341–358
Hara H, Yamamoto Y, Higashitani A et al (1991) Cloning, mapping, and characterization of the Escherichia coli prc gene, which is involved in C-terminal processing of penicillin-binding protein 3. J Bacteriol 173:4799–4813
Hessner MJ, Wejksnora PJ, Collins MLP (1991) Construction, characterization, and complementation of Rhodospirillum rubrum puf region mutants. J Bacteriol 173:5712–5722
Kiley PJ, Donohue TJ, Havelka WA et al (1987) DNA sequence and in vitro expression of the B875 light-harvesting polypeptides of Rhodobacter sphaeroides. J Bacteriol 169:742–750
Link AJ, Phillips D, Church GM (1997) Methods for generating precise deletions and insertions in the genome of wild-type Escherichia coli: application to open reading frame characterization. J Bacteriol 179:6228–6237
Masuda S, Bauer CE (2004) Null mutation of HvrA compensates for loss of an essential relA/spoT-like gene in Rhodobacter capsulatus. J Bacteriol 186:235–239
Mueller PR, Collins MLP (1983) Identification of two distinct lactate dehydrogenases in Rhodospirillum rubrum. J Bacteriol 153:1562–1566
Myers CR, Collins MLP (1986) Cell-cycle-specific oscillation in the composition of chromatophore membrane in Rhodospirillum rubrum. J Bacteriol 166:818–823
Myers CR, Collins MLP (1987) Cell-cycle-specific fluctuation in cytoplasmic membrane composition in aerobically grown Rhodospirillum rubrum. J Bacteriol 169:5445–5451
Oelmuller R, Herrmann RG, Pakrasi HB (1996) Molecular studies of CtpA, the carboxyl-terminal processing protease for the D1 protein of the Photosystem II reaction center in higher plants. J Biol Chem 271:21848–21852
Raiger-Iustman LJ, Kerber NL, Pucheu NL, Bornmann MJ, Kohler S, Labahn A, Tadros M, Drews G, Garcia AF (2006) Characterization of a mutant strain of Rhodovulum sulfidophilum lacking the pufA and pufB genes encoding the polypeptides for the light-harvesting complex 1(B870). Arch Microbiol 185:407–415
Reiling SA, Jansen JA, Henley BJ et al (2005) Prc protease promotes mucoidy in mucA mutants of Pseudomonas aeruginosa. Microbiology 151:2251–2261
Sambrook J, Russell DW (2001) Molecular cloning a laboratory manual, 3rd edn. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York
Schagger H, Von Jagow G (1987) Tricine–sodium dodecyl sulfate–polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa. Anal Biochem 166:368–379
Tadros MH, Drews G (1990) Pigment-proteins of antenna complexes from non-sulfur bacteria: localization in the membrane, alignments of primary structure and structural predictions. In: Drews G, Dawes EA (eds) Molecular biology of membrane-bound complexes in phototrophic bacteria. Plenum, New York, pp 181–192
Yano T, Sanders C, Catalano J et al (2005) sacB-5-Fluoroorotic Acid-pyrE-based bidirectional selection for integration of unmarked alleles in the chromosome of Rhodobacter capsulatus. Appl Environ Microbiol 71:3014–3024
Youvan DC, Bylina EJ, Alberti M et al (1984) Nucleotide and deduced polypeptide sequences of the photosynthetic reaction-center, B870 antenna, and flanking polypeptides from R. capsulata. Cell 37:949–957
Yuan JS, Reed A, Chen F, Stewart CN (2006) Statistical analysis of real-time PCR data. BMC Bioinf 7:85–96
Author information
Authors and Affiliations
Corresponding author
Additional information
Nicholas C. Butzin and Jodi A. Benson contributed equally to this research.
Electronic supplementary material
Below is the link to the electronic supplementary material.
Rights and permissions
About this article
Cite this article
Butzin, N.C., A. Benson, J., Merchant, D.J. et al. Role of the PufB and PufA C-Terminal Extensions in the Assembly of Rhodospirillum rubrum Light-Harvesting Antenna. Curr Microbiol 60, 301–306 (2010). https://doi.org/10.1007/s00284-009-9541-5
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s00284-009-9541-5