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Pseudomonas oleovorans subsp. lubricantis subsp. nov., and Reclassification of Pseudomonas pseudoalcaligenes ATCC 17440T as Later Synonym of Pseudomonas oleovorans ATCC 8062T

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Abstract

Isolate RS1T isolated from used metalworking fluid was found to be a Gram-negative, motile, and non-spore forming rod. Based on phylogenetic analyses with 16S rRNA, isolate RS1T was placed into the mendocina sublineage of Pseudomonas. The major whole cell fatty acids were C18:1ω7c (32.6%), C16:0 (25.5%), and C15:0 ISO 2OH/C16:1ω7c (14.4%). The sequence similarities of isolate RS1T based on gyrB and rpoD genes were 98.9 and 98.0% with Pseudomonas pseudoalcaligenes, and 98.5 and 98.1% with Pseudomonas oleovorans, respectively. The ribotyping pattern showed a 0.60 similarity with P. oleovorans ATCC 8062T and 0.63 with P. pseudoalcaligenes ATCC17440T. The DNA G + C content of isolate RS1T was 62.2 mol.%. The DNA–DNA relatedness was 73.0% with P. oleovorans ATCC 8062T and 79.1% with P. pseudoalcaligenes ATCC 17440T. On the basis of morphological, biochemical, and molecular studies, isolate RS1T is considered to represent a new subspecies of P. oleovorans. Furthermore, based on the DNA–DNA relatedness (>70%), chemotaxonomic, and molecular profile, P. pseudoalcaligenes ATCC 17440T and P. oleovorans ATCC 8062T should be united under the same name; according to the rules of priority, P. oleovorans, the first described species, is the earlier synonym and P. pseudoalcaligenes is the later synonym. As a consequence, the division of the species P. oleovorans into two novel subspecies is proposed: P. oleovorans subsp. oleovorans subsp. nov. (type strain ATCC 8062T = DSM 1045T = NCIB 6576T), P. oleovorans subsp. lubricantis subsp. nov. (type strain RS1T = ATCC BAA-1494T = DSM 21016T).

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Acknowledgments

We would like to extend our sincere thanks to Dr. Jeff Landgraf and Joseph Leykam of RTSF at Michigan State University, Michigan, USA for the determination of % G + C. We would also like to thank Dr. Ellen Dickstein at University of Florida, Gainesville, Florida, USA for FAME analysis, Dr. Stacie Frye at Bacterial Barcodes, Inc., Athens, Georgia, USA for Rep-PCR analysis and Bettina Sträubler at DSMZ for her excellent technical support.

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Authors and Affiliations

  1. Biological Sciences, Michigan Technological University, Houghton, MI, USA

    Ratul Saha & Susan Bagley

  2. Molecular Ecology and Systematics, DSMZ-Deutsche Sammlung von Mikrooganismen und Zellkulturen GmbH, Braunschweig, Germany

    Cathrin Spröer

  3. Bacteriology, American Type Culture Collection, Manassas, VA, USA

    Brian Beck

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  1. Ratul Saha
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  2. Cathrin Spröer
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  3. Brian Beck
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  4. Susan Bagley
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Correspondence to Ratul Saha.

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Saha, R., Spröer, C., Beck, B. et al. Pseudomonas oleovorans subsp. lubricantis subsp. nov., and Reclassification of Pseudomonas pseudoalcaligenes ATCC 17440T as Later Synonym of Pseudomonas oleovorans ATCC 8062T. Curr Microbiol 60, 294–300 (2010). https://doi.org/10.1007/s00284-009-9540-6

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  • DOI: https://doi.org/10.1007/s00284-009-9540-6

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