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Nocardioides dilutes sp. nov. Isolated from Soil in Bigeum Island, Korea

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Abstract

A short coccoid-rod-shaped, nonmotile actinobacteria strain MSL-11T was isolated from soil in Bigeum Island, Korea. A polyphasic study was undertaken to establish the taxonomic position of this strain. Phylogenetic analysis based on the 16S rRNA gene sequence revealed that strain MSL-11T forms an evolutionary lineage within the radiation of the genus Nocardioides. The cell wall peptidoglycan of strain MSL-11T contained ll-diaminopimelic acid, indicating wall chemotype I. The predominant menaquinone was MK-8(H4). Strain MSL-11T had a cellular fatty acid profile containing straight-chain, branched, unsaturated, and 10-methyl fatty acids, with iso-C16:0 as a major fatty acid component detected. The DNA G + C content of the strain was 71.8 mol%. Comparative 16S rRNA gene sequencing revealed that the strains constituted a distinct subclade within the genus Nocardioides, displaying a 16S rRNA gene sequence similarity of about 95.68% with Nocardioides jensenii DSM 20641T. On the basis of both phenotypic and phylogenetic evidence, the strain is separated from previously described Nocardioides species and should be assigned to represent a novel species of the genus Nocardioides, for which the name Nocardioides dilutes sp. nov. is proposed. The type strain is strain MSL-11T (= KCTC 19288T = DSM 19318T).

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Acknowledgments

This work was supported by the 21C Frontier Microbial Genomics and Application Centre Program Korean Ministry of Science & Technology (MOST) and Korea Foundation for International Cooperation of Science & Technology (KICOS) through a grant provided by MOST in the Global Partnership Program (No. M60602000001-06E0200-00100), Republic of Korea.

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  1. Functional Metabolomics Research Center, KRIBB, Daejeon, 305-806, Korea

    Syed G. Dastager, Jae-Chan Lee, Yoon-Jung Ju, Dong-Jin Park & Chang-Jin Kim

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  1. Syed G. Dastager
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  2. Jae-Chan Lee
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  3. Yoon-Jung Ju
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  4. Dong-Jin Park
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  5. Chang-Jin Kim
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Correspondence to Chang-Jin Kim.

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Dastager, S.G., Lee, JC., Ju, YJ. et al. Nocardioides dilutes sp. nov. Isolated from Soil in Bigeum Island, Korea. Curr Microbiol 56, 569–573 (2008). https://doi.org/10.1007/s00284-008-9125-9

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  • DOI: https://doi.org/10.1007/s00284-008-9125-9

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