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Tip-α (hp0596 Gene Product) Is a Highly Immunogenic Helicobacter pylori Protein Involved in Colonization of Mouse Gastric Mucosa

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Abstract

A product of the Helicobacter pylori hp0596 gene (Tip-α) is a highly immunogenic homodimeric protein, unique for this bacterium. Cell fractionation experiments indicate that Tip-α is anchored to the inner membrane. In contrast, the three-dimensional model of the protein suggests that Tip-α is soluble or, at least, largely exposed to the solvent. hp0596 gene knockout resulted in a significant decrease in the level of H. pylori colonization as measured by real-time PCR assay. In addition, the Tip-α recombinant protein was determined to stimulate macrophage to produce IL-1α and TNF-α. Both results imply that Tip-α is rather loosely connected to the inner membrane and potentially released during infection.

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Authors and Affiliations

  1. Department of Bacterial Genetics, Institute of Microbiology, University of Warsaw, ul. Miecznikowa 1, 02-096, Warsaw, Poland

    Renata Godlewska & Elżbieta K. Jagusztyn-Krynicka

  2. Laboratory of Bioinformatics and Protein Engineering, International Institute of Cell and Molecular Biology, ul. ks. Trojdena 4, 02-109, Warsaw, Poland

    Marcin Pawlowski

  3. Department of Gastroenterology, Medical Center for Postgraduate Education, Maria Sklodowska-Curie Memorial Cancer Center, and Institute of Oncology, 02-781, Warsaw, Poland

    Artur Dzwonek, Michal Mikula & Jerzy Ostrowski

  4. Department of Immunology, Institute of Zoology, University of Warsaw, ul. Miecznikowa 1, 02-096, Warsaw, Poland

    Nadzieja Drela

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  1. Renata Godlewska
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  2. Marcin Pawlowski
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  4. Michal Mikula
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  7. Elżbieta K. Jagusztyn-Krynicka
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Correspondence to Renata Godlewska.

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Godlewska, R., Pawlowski, M., Dzwonek, A. et al. Tip-α (hp0596 Gene Product) Is a Highly Immunogenic Helicobacter pylori Protein Involved in Colonization of Mouse Gastric Mucosa. Curr Microbiol 56, 279–286 (2008). https://doi.org/10.1007/s00284-007-9083-7

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  • DOI: https://doi.org/10.1007/s00284-007-9083-7

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