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Berberine Inhibits Arylamine N-Acetyltransferase Activity and Gene Expression in Salmonella Typhi

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Abstract

The effects of berberine on growth, arylamine N-acetyltransferase (NAT) activity, and gene expression in Salmonella Typhi (Typhi) were described. The growth inhibition of Typhi was determined by measuring absorbance by optical density (OD at 650 nm). The NAT activity was determined by measuring the levels of 2-aminofluorene (AF) and N-acetyl-2-aminofluorene (AAF) by high-performance liquid chromatography. The results demonstrated that 24-h berberine treatment decreased bacteria growth and amounts of AAF in Typhi. Western blotting and flow cytometry were used for examining the levels of NAT after bacteria were cotreated with or without various concentrations of berberine, and results indicated that berberine decreased the levels of NAT in Typhi. Polymerase chain reaction was used for examining the gene expression of NAT (mRNA NAT), and results indicated that berberine affects mRNA NAT1 expression in Typhi.

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Acknowledgments

This work was supported by grant CMU92-M-26 from China Medical University.

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Authors and Affiliations

  1. Department of Microbiology, China Medical University, Taichung, 404, Taiwan, Republic of China

    Lii-Tzu Wu & Jing-Gung Chung

  2. Department of Internal Medicine, China Medical University Hospital, 404, Taichung, Taiwan, Republic of China

    Mei-Fen Tsou

  3. Department of Nursing, Chungtai Institute of Health Sciences and Technology, 11 Pro-Tze Lang, 40605, Takun, Taichung, Taiwan, Republic of China

    Chin-Chin Ho

  4. Department of Medical Laboratory Science and Biotechnology, China Medical University, Taichung, 404, Taiwan, Republic of China

    Jing-Yuan Chuang

  5. Department of Parasitology, China Medical University, Taichung, 404, Taiwan, Republic of China

    Hsiu-Maan Kuo

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  1. Lii-Tzu Wu
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Correspondence to Jing-Gung Chung.

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Wu, LT., Tsou, MF., Ho, CC. et al. Berberine Inhibits Arylamine N-Acetyltransferase Activity and Gene Expression in Salmonella Typhi. Curr Microbiol 51, 255–261 (2005). https://doi.org/10.1007/s00284-005-4569-7

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  • DOI: https://doi.org/10.1007/s00284-005-4569-7

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