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Construction of Secretory Expression System Suitable to Express Glucagon Under the Control of PL Promoter

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Abstract

It was reported that PL promoter and alkaline phosphatase (phoA) signal peptide were used to construct secretory expression plasmid suitable to express glucagon and [Des-His1] glucagon in E. coli BL21 herein. Expression studies showed these two peptides could be expressed and secreted into the culture medium. The expression yield of recombinant glucagon reached 3.46 mg/L/OD600 unit of cells in shake flask. The yield of [Des-His1] glucagon was found to be higher than that of glucagon. In addition, some factors involved in secretion were studied too.

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Authors and Affiliations

  1. Institute of Biochemistry and Cell Biology, Shanghai Institute for Biological Sciences, Chinese Academy of Sciences, 320 Yueyang Road, Shanghai, 200031, People’s Republic of China, CN

    Chongwei Wen, Renbao Gan & Shangquan Zhu

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  1. Chongwei Wen
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  2. Renbao Gan
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  3. Shangquan Zhu
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Received: 26 September 2002 / Accepted: 24 October 2002

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Wen, C., Gan, R. & Zhu, S. Construction of Secretory Expression System Suitable to Express Glucagon Under the Control of PL Promoter. Curr Microbiol 47, 0180–0185 (2003). https://doi.org/10.1007/s00284-002-3988-y

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  • DOI: https://doi.org/10.1007/s00284-002-3988-y

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