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Silencing CD147 inhibits tumor progression and increases chemosensitivity in murine lymphoid neoplasm P388D1 cells

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Abstract

Overexpression of extracellular matrix metalloproteinase inducer (EMMPRIN or CD147), a member glycoprotein enriched on the surface of many malignant tumor cells, promotes tumor progression and confers resistance to some chemotherapeutic drugs. To investigate the possible role of CD147 in the macrophage-like lymphoid neoplasm P388D1 cells progression, we used RNA interference approach to silence CD147 expression. The results showed that silencing of CD147 in P388D1 cells impeded the expression of MMP11 at both mRNA and protein levels. The reduced CD147 expression also resulted in reductions in tumorigenicity, as well as decreased in regional lymph node metastasis. Furthermore, the down-regulation of CD147 expression sensitized cells to be more sensitive to chemotherapeutic drugs. Treatment of tumor cells with U-0126, an inhibitor of mitogen-activated protein kinase/Erk, also down-regulated the expression of MMP11. Our current results indicate that the expression of CD147 functionally mediates tumor progression and is a potential target for therapeutic anti-cancer drugs.

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Acknowledgment

This work was supported by grants from National Natural Science Foundation of China (30670466).

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Authors and Affiliations

  1. Department of Biochemistry, Institute of Glycobiology, Dalian Medical University, 9 South Lvshun Road, Dalian, 116044, Liaoning Province, China

    Li Jia & Jianing Zhang

  2. College of Laboratory Medicine, Dalian Medical University, Dalian, 116044, Liaoning Province, China

    Li Jia, Wei Wei, Henggui Xu & Xiaoyan Miao

  3. Department of Toxicology, Dalian Medical University, Dalian, 116044, Liaoning Province, China

    Jun Cao

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  1. Li Jia
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  2. Wei Wei
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  3. Jun Cao
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  5. Xiaoyan Miao
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  6. Jianing Zhang
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Correspondence to Jianing Zhang.

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Jia, L., Wei, W., Cao, J. et al. Silencing CD147 inhibits tumor progression and increases chemosensitivity in murine lymphoid neoplasm P388D1 cells. Ann Hematol 88, 753–760 (2009). https://doi.org/10.1007/s00277-008-0678-2

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  • DOI: https://doi.org/10.1007/s00277-008-0678-2

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