Abstract
The human neutrophil antigens HNA-1a, -1b and -1c play an important role in immune neutropenia. The frequencies of the coding FCGR3B genes were determined in different populations. New FCGR3B variants were also found in some populations. This study investigated the FCGR3B gene frequencies and FCGR3 variants in a Chinese population compared with the results of Northern Germans and African Blacks (Uganda). Our results show that the gene frequencies in 413 healthy Chinese individuals from Zhejiang Province were 0.565 for FCGR3B*1, 0.430 for FCGR3B*2 and 0.00 for FCGR3B*3. The genotype frequency of FCGR3B null was 0.48% (2/413). Sequencing of FCGR3 revealed that in seven out of 19 Chinese individuals, cloned and sequenced DNA fragments that exhibited variants caused by single nucleotide exchanges at one or more of the polymorphic positions 141, 147, 227, 266 and 277 in exon 3 also existed in this Chinese population. From the present study, it is concluded that the FCGR3B*1 gene is more frequent in a Chinese population from Zhejiang Province than the FCGR3B*2 gene, and the FCGR3B*3 gene seems to be absent, which is in contrast to studies in the white populations. Gene variants caused by single nucleotide exchanges were found in addition to the well-known forms, but the reason for this remains unclear.
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References
Ravetch JV, Kinet JP (1991) Fc receptors. Annu Rev Immunol 9:457–492
Bux J (1999) Nomenclature of granulocyte alloantigens. Transfusion 39:662–663
Ory PA, Clark MR, Kwoh EE, Clarkson SB, Goldstein IM (1989) Sequences of complementary DNAs that encode the NA1 and NA2 forms of Fc receptor III on human neutrophils. J Clin Invest 84:1688–1691
Lalezari P, Radel E (1974) Neutrophil-specific antigens. Immunology and clinical significance. Semin Haematol 11:281–290
Bux J (1999) Granulocyte antibody-mediated neutropenias and transfusion reactions. Infusionsther Transfusionsmed 26:152–157
Bierling P, Poulet E, Fromont P, Seror T, Bracq C, Duedari N (1990) Neutrophil-specific antigen and gene frequencies in the French population (letter). Transfusion 30:848–849
Kissel K, Hofmann C, Gittinger FS, Daniels G, Bux J (2000) HNA-1a, HNA-1b, and HNA-1c (NA1, NA2, SH) frequencies in African and American Blacks and in Chinese. Tissue Antigen 56:143–148
Flesch BK, Doose S, Siebert R, Ntambi E, Neppert J (2002) FCGR3 variants and expression of human neutrophil antigen-1a, -1b, and -1c in the population of northern Germany and Uganda. Transfusion 42:469–475
Hessner MJ, Curtis BR, Endean DJ, Aster RH (1996) Determination of neutrophil antigen gene frequencies in five ethnic groups by polymerase chain reaction with sequence-specific primers. Transfusion 36:895–899
Ohto H, Matsuo Y (1989) Neutrophil-specific antigens and gene frequencies in Japanese (letter). Transfusion 29:654
Bux J, Stein EL, Bierling P, Fromont P, Clay M, Stroncek D, Santoso S (1997) Characterization of a new alloantigen (SH) on the human neutrophil Fcγreceptor IIIb. Blood 89:1027–1034
de Haas M, Kleijer M, van Zwieten R, Roos D, von dem Borne AEGKr (1995) Neutrophil FcγRIIIb deficiency, nature and clinical consequences: a study of 21 individuals from 14 families. Blood 86:2403–2413
Matsuo K, Procter J, Stroncek D (2000) Variations in genes encoding neutrophil antigens NA1 and NA2. Transfusion 40:645–653
Bux J, Stein EL, Santoso S, Mueller-Eckhardt C (1995) NA gene frequencies in the German population, determined by polymerase chain reaction with sequence-specific primers. Transfusion 35:54–57
Steffensen R, Gülen T, Varming K, Jersild C (1999) FcγRIIIb polymorphism: evidence that NA1/NA2 and SH are located in two closely linked loci and that the SH allele is linked to the NA1 allele in the Danish population. Transfusion 39:593–598
Sambrook J, Fritsch EF, Maniatis T (eds) (1989): Molecular Cloning. A laboratory Manual, 2nd edn, Cold Spring Harbor Laboratory Press, New York, pp 1.85
Lin M, Chen CC, Wang CL, Lee HL (1994) Frequencies of neutrophil- specific antigens among Chinese in Taiwan (letter). Vox Sang 66:247
Muniz-Diaz E, de Haas M, Madoz P, Koene HR, Kleijer M, Arilla M, Gracia M, Puig LL, von dem Borne AEGKr (1996) High frequency of the neutrophil FcγRIIIb deficiency in Spain. A study of 20 individuals from 4 families. Transfusion Med 6:194
Fromont P, Bettaieb A, Skouri H, Floch C, Poulet E, Duedari N, Bierling P (1992) Frequency of the polymorphonuclear neutrophil Fc gamma receptor III deficiency in the French population and its involvement in the development of neonatal alloimmune neutropenia. Blood 79:2131–2134
Flesch BK, Achtert G, Bauer F, Neppert J (1998) The NA "Null" phenotype of a young man is caused by an FcγRIIIB gene deficiency while the products of the neighboring FcγRIIA and FcγRIIIA genes are present. Ann Hematol 76:215–220
Huizinga TWJ, Kuijper RWAM, Kleijer M, Schulpen TWJ, Cuypers TM, Roos D, von dem Borne AEGKr (1990) Maternal genomic neutrophil FcγRIII deficiency leading to neonatal isoimmune neutropenia. Blood 76:1927–1932
Stroncek D, Bux J (2002) Is it time to standardize granulocyte alloantigen nomenclature? Transfusion 42:393–395
Lalezari P (2002) Nomenclature for neutrophil-specific antigens. Transfusion 42:1396–1397
Matsuo K, Procter JL, Chanock S, Stroncek DF (2001) The expression of NA antigens in people with unusual Fcγ receptor III genotypes. Transfusion 41:775–782
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Tong, Y., Jin, J., Yan, L. et al. FCGR3B gene frequencies and FCGR3 variants in a Chinese population from Zhejiang Province. Ann Hematol 82, 574–578 (2003). https://doi.org/10.1007/s00277-003-0725-y
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DOI: https://doi.org/10.1007/s00277-003-0725-y