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Micro-computed tomography study of the subchondral bone of the vertebral endplates in a porcine model: correlations with histomorphometric parameters

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Abstract

Purpose

Subchondral bone (SCB) of the vertebral endplates (VEP) is the principal site of changes in vertebral trabecular microarchitecture secondary to intervertebral disc degeneration. However, the microstructure of this region has not yet been clearly characterized.

Methods

One thoracic and one lumbar vertebral unit (vertebra-disc-vertebra) was removed in nine pigs aged 4 months. Three samples (one central and two laterals) were taken from each VEP. Micro-CT examination and histomorphometric measurements of the subchondral trabecular bone of the VEP were carried out. Correlations between micro-CT and histological parameters were sought.

Results

Trabecular network was significantly denser [increased bone volume fraction (BV/TV) and trabecular number (Tb.N), decreased intertrabecular separation (Tb.Sp)] in the cranial endplates of the vertebral units. It was also significantly denser and less well organized [increased degree of anisotropy (DA)] in the centre of the VEP. The thickness of the cartilage endplate (CEP), SCB and growth cartilage were significantly lower in the centre of the VEP. There was a significant negative correlation between BV/TV, Tb.N and DA with the thicknesses of the CEP and SCB whereas Tb.Sp was positively correlated with these two parameters.

Conclusion

We observed densification of the trabecular network in the centre of the VEP overlying the nucleus pulposus, partly related to thinner hyaline cartilage. Densification is associated with more anisotropic architecture that could cause lower mechanical strength in this area. This study provides new information on the microarchitecture of the SCB of the VEP which will make it possible to validate future models.

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Acknowledgments

The authors thank Pr Jean Puget for his technical support. All experiments were carried out at the Ecole Nationale Vétérinaire, Lyon, France, in accordance with current regulations on the care and use of experimental animals.

Conflict of interest statement

The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. Service de Chirurgie Orthopédique et Traumatologique, Institut Locomoteur, Centre Hospitalier Universitaire Rangueil, 1, avenue Jean Poulhès, TSA 50032, 31059, Toulouse Cedex 9, France

    Jean-Michel Laffosse

  2. Laboratoire de Biomécanique, Centre Hospitalier Universitaire Purpan, Toulouse, France

    Jean-Michel Laffosse, Franck Accadbled, Jérôme Sales de Gauzy & Pascal Swider

  3. Service de Chirurgie Orthopédique Pédiatrique, Hôpital des Enfants, Toulouse, France

    Charles Kinkpe, Franck Accadbled & Jérôme Sales de Gauzy

  4. Laboratoire d’Anatomo-Cytopathologie, Centre Hospitalier Universitaire Rangueil, Toulouse, France

    Anne Gomez-Brouchet

  5. Ecole Nationale Vétérinaire de Lyon, Lyon, France

    Eric Viguier

Authors
  1. Jean-Michel Laffosse
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  2. Charles Kinkpe
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  3. Anne Gomez-Brouchet
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  4. Franck Accadbled
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  5. Eric Viguier
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  6. Jérôme Sales de Gauzy
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  7. Pascal Swider
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Corresponding author

Correspondence to Jean-Michel Laffosse.

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Laffosse, JM., Kinkpe, C., Gomez-Brouchet, A. et al. Micro-computed tomography study of the subchondral bone of the vertebral endplates in a porcine model: correlations with histomorphometric parameters. Surg Radiol Anat 32, 335–341 (2010). https://doi.org/10.1007/s00276-009-0569-9

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  • DOI: https://doi.org/10.1007/s00276-009-0569-9

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